ELISA

Question 1

What does ELISA ?

Answer 1

Enzyme linked immunosorbant assay

Question 2

What does ELISA use?

Answer 2

Antibodies to detect the presence of an antigen within a solution

Question 3

What are the 3 forms of ELISA?

Answer 3

Direct
Indirect
Sandwich

Question 4

Describe direct ELISA

Answer 4

The antigen is allowed to bind to the surface of a multiwell plate. A primary antibody, linked to a reporter enzyme, is added to the well and binds to the antigen

Question 5

Describe indirect ELISA

Answer 5

The antigen is allowed to bind to the surface of a multiwell plate. A primary antibody is added to the well and allowed to bind to the antigen. A secondary antibody, linked to a reporter enzyme, is then added, which binds to the primary antibody

Question 6

Describe sandwich ELISA

Answer 6

A capture antibody is bound to the surface of a multiwell plate. The antigen is added and allowed to bind to the capture antibody. A primary antibody, which binds to the antigen, is added to the well. A secondary antibody, linked to a reporter enzyme, is then added, which binds to the primary antibody