Protein Determination Flashcards
Amount of Protein in Urine normally
10 mg/dl or 100mg/24 hrs
Presence of Increased Amount of Protein in urine is termed as?
Proteinuria
Primary Indicator of Renal Disease
Protein
Proteinuria is a result of?
Bot reabsorbed protein in the tubules
2 Ways to Detect Urinary Proteins
- qualitatively
- semi quantitatively
Protein is denatured upon exposure to extremes of pH or temp
Protein Precipitation Techniques
4 Protein Precipitation Techniques
- Heat and Acetic Acid Test
- Sulfosalicylic Acid Test
- Robert’s Test
- Heller’s Ring Test
Positive Results for Protein Precipitation Techniques
Cloudiness or Ring at Junction of Reagents
Most commonly used test done at room temperature
Sulfosalicylic Acid Test
Principle of Reagent Strip for Proteins
Protein Error of Indication
What causes for reagent strip to change color for the detection of protein?
when Hydrogen Ions meet Anions (Proteins)
Volume of Urine and Reagent for Robert’s Test
3cc / 3cc
Volume of reagent for Heller’s Ring Test?
2 cc of HNO3
Volume of urine and reagent for sulfosalicylic acid test
5cc Urine / 2cc 3% Aqueous SSA Solution
Volume of Urine and Reagent for Purdy’s Test
10 cc Urine / 2 cc Saturated Solution of NaCl / 2-5 Drops of Glacial Acetic Acid
