Glucose Determination

Question 1

Normal Urine contains sugar volume of?

Answer 1

<0.1 mg/dl

Question 2

Main Sugar Detected

Answer 2

Glucose

Question 3

High concentration of glucose in urine is known as?

Answer 3

Glucosuria

Question 4

Glucosuria is associated with with what diseases? (5)

Answer 4

1. DM
2. Impaired Tubular Reabsorption
3. CNS Damage
4. Thyroid Disorders
5. Pregnancy with Latent DM

Question 5

Copper Reduction Tests (3)

Answer 5

1. Benedict’s
2. Fehling’s
3. Clinitest

Question 6

Other Reducing Sugars (3)

Answer 6

1. Lactose
2. Maltose
3. Galactose

Question 7

Principle of Copper Reduction Tests

Answer 7

Reduce Copper Sulfate to Cuprous Oxide by Heat and Alkali

Question 8

Reagent Strip utilizes what reaction?

Answer 8

Glucose Oxidase Reaction

Question 9

The test area is impregnated with a mixture of? (4)

Answer 9

1. Glucose Oxidase
2. Peroxidase
3. Chromogen
4. Buffer

Question 10

The catalytic reaction of glucose oxidase between glucose and oxygen produces? (2)

Answer 10

1. Gluconic Acid
2. Peroxide

Question 11

The enzyme peroxidase will catalyze the reaction between? (2)

Answer 11

1. Peroxide
2. Chromogen

Question 12

Volume of Urine and Reagent in Benedict’s Test

Answer 12

2.5 cc Reagent / 4 Drops Urine

Question 13

How long will you boil the solution in Benedict’s Test?

Answer 13

5 Mins

Question 14

Color Grading and Reporting of Benedicts Test

Answer 14

1. Blue - Nil
2. Green - Trace
3. Brown - 2+
4. Yellow-Orange - 3+
5. Brick Red - 4+

Question 15

Volume of Urine and Reagent for Fehling’s Test

Answer 15

2 cc A & B Solution / 4 cc DH2O / 1cc Urine

Question 16

Volume of Urine and Reagent in Clinitest Tablets

Answer 16

5 Drops Urine / 10 Drops Water