prokaryotic transcription Flashcards
transcription unit is …
the DNA sequence extending from promotor to the termination region
the promoter is …
the sequence of DNA to which proteins bind to initiate transcription
primary transcript is …
the initial product of transcription by RNA polymerase
“processivity” means …
the ability of an enzyme to catalyze consecutive reactions without releasing its substrate
the 3 steps of prokaryotic transcription
initiation
elongation
termination
initiation begins with …
the binding of RNA poly holoenzyme to the promoter sequences in the template DNA
elongation …
propagates the mRNA synthesis
termination is …
stopping RNA synthesis at termination sequences
the major enzyme associated with prokaryotic transcription is …
RNA polymerase
supercoils may develop once … and can be fixed by …
sigma factor is released after initiation
fixed by topoisomerase
the RNA-DNA hybrid region (forming at the promoter region) during elongation functions to …
hold the mRNA transcript to the DNA
two types of termination
Rho factor dependent
Rho factor independent (when Rho is not involved)
Rho independent termination of prokaryotic transcription steps
self-complementary RNA
|
V
RNA folds back
|
GC rich hairpin loop
|
stops RNA poly
|
weak A-U binding causes RNA to separate from DNA
the Rho factor is structured as a … and is a …
hexamer
ATPase with helicase activity
clinically, prokaryotic transcription has been targeted to design …
antibiotics to prevent bacterial cell growth and viral proliferation
