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Molecular Biology > Prokaryotic Replication > Flashcards

Prokaryotic Replication Flashcards

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1
Q

In what direction are Okazaki fragments synthesised?

A

5’ to 3’

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2
Q

How long are Okazaki fragments in prokaryotes?

A

1000-2000

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3
Q

What length are Okazaki fragments in eukaryotes?

A

100-200

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4
Q

What activities does Pol3 carry out?

A

5’ to 3’ polymerase

3’ to 5’ exonuclease (proofreading?

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5
Q

What unit helps increase the processivity of DNA polymerase?

A

Beta Clamp

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6
Q

What loads the beta clamp?

A

Gamma complex

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7
Q

Name the units and function of Pol3

A

alpha - 5’ - 3’ polymerase
ε - 3’ - 5’ exonuclease
θ - helps load ε

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8
Q

What are the tau sub units for in Pol 3?

A

dimerise the pol 3 complex for each strand

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9
Q

What is the replisome?

A

Large protein complex that carries out DNA replication

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10
Q

Evidence for semi conservative replication?

A

Hybrid DNA is formed using N15, centrifuge shows that the hybrid dna is a mix between N14 and N15

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11
Q

How is each tNTP added?

A

The 3’ OH attacks the alpha phosphate of the tNTP forming a pyrophosphate

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12
Q

What is DnaG also known as?

A

DNA Primase

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13
Q

What does DNA primase do?

A

Forms a 5-10bp RNA primer

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14
Q

Pol 1 (PolA) carries out which functions

A

5’ - 3’ polymerase
5 - 3’ exonuclease (removing RNA primer)
3’ - 5’ proofreading

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15
Q

What do single stranded binding proteins do?

A

SSBs bind to single stranded bits of DNA to stabilise them. They are an example of COOP binding.

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16
Q

Function of DnaC?

A

To load DnaB/helicase

17
Q

What are the types of topoisomerases? and their function?

A

Topo1 - knicks DNA to release tension

Topo2 - unknots DNA

18
Q

Name of DNA topo 2 in E. Coli?

A

DNA gyrase

19
Q

Name of site of initiation in E.Coli?

A

oriC

20
Q

What nucleotide is rich in initiator sequence?

A

AT

21
Q

How does termination occur?

A

Ter sites (theres ones for each direction) forces forks to stop. Tus proteins bind and impede DNA helicase

Molecular Biology (7 decks)

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