Eukaryotic Replication

Question 1

Stages of Eukaryotic cell division?

Answer 1

G1 - Gap 1
S - DNA Synthesis
G2 - Gap 2
M - Mitosis

Question 2

What is ARS?

Answer 2

Autonomic Replicative Sequences

Question 3

What is ORE?

Answer 3

Origin of Replication Element

Question 4

ORE binds to DnaA like protein to form…?

Answer 4

Origin of Replication Complex

Question 5

How big are replication bubbles?

Answer 5

30-300kbp

Question 6

What stage is a new licensing factor synthesised?

Answer 6

G2

Question 7

What are the licensing factors in humans?

Answer 7

Cdc6 and cdt1

Question 8

What is the eukaryotic equivalent to DnaB?

Answer 8

MCM (minichromosome maintenance)

Question 9

Name of the eukaryotic sliding clamp?

Answer 9

PCNA

Question 10

What shape is PCNA?

Answer 10

homotrimeric

Question 11

What shape is the Beta clamp in prokaryotes?

Answer 11

dimeric

Question 12

What fold are the sliding clamps?

Answer 12

6 fold

Question 13

What is the name of the clamp loader in eukaryotes?

Answer 13

Replication Factor C (RFC)

Question 14

Shape of clamp loaders?

Answer 14

Pentameric

Question 15

What is the name of the eukaryotic SSB?

Answer 15

Replication protein A (RPA1,2,3) [heterotrimer]

Question 16

What is the name of eukaryotic primase?

Answer 16

Pol alpha

Question 17

What is the name of the prokaryotic primer?

Answer 17

DnaG

Question 18

Gene used to create telomeres?

Answer 18

hTERT

Question 19

Why is there telomerases?

Answer 19

RNA primer needs to be produced for the lagging strand?

Question 20

What happens to telomeres during ageing?

Answer 20

Shorten

Question 21

How does a cell know which strand is original?

Answer 21

Methylation

Question 22

How does proofreading work?

Answer 22

Incorrect nucleotides have a higher affinity for exonuclease active site