Eukaryotic Replication Flashcards
Stages of Eukaryotic cell division?
G1 - Gap 1
S - DNA Synthesis
G2 - Gap 2
M - Mitosis
What is ARS?
Autonomic Replicative Sequences
What is ORE?
Origin of Replication Element
ORE binds to DnaA like protein to form…?
Origin of Replication Complex
How big are replication bubbles?
30-300kbp
What stage is a new licensing factor synthesised?
G2
What are the licensing factors in humans?
Cdc6 and cdt1
What is the eukaryotic equivalent to DnaB?
MCM (minichromosome maintenance)
Name of the eukaryotic sliding clamp?
PCNA
What shape is PCNA?
homotrimeric
What shape is the Beta clamp in prokaryotes?
dimeric
What fold are the sliding clamps?
6 fold
What is the name of the clamp loader in eukaryotes?
Replication Factor C (RFC)
Shape of clamp loaders?
Pentameric
What is the name of the eukaryotic SSB?
Replication protein A (RPA1,2,3) [heterotrimer]