Eukaryotic Replication Flashcards

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1
Q

Stages of Eukaryotic cell division?

A

G1 - Gap 1
S - DNA Synthesis
G2 - Gap 2
M - Mitosis

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2
Q

What is ARS?

A

Autonomic Replicative Sequences

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3
Q

What is ORE?

A

Origin of Replication Element

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4
Q

ORE binds to DnaA like protein to form…?

A

Origin of Replication Complex

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5
Q

How big are replication bubbles?

A

30-300kbp

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6
Q

What stage is a new licensing factor synthesised?

A

G2

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7
Q

What are the licensing factors in humans?

A

Cdc6 and cdt1

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8
Q

What is the eukaryotic equivalent to DnaB?

A

MCM (minichromosome maintenance)

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9
Q

Name of the eukaryotic sliding clamp?

A

PCNA

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10
Q

What shape is PCNA?

A

homotrimeric

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11
Q

What shape is the Beta clamp in prokaryotes?

A

dimeric

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12
Q

What fold are the sliding clamps?

A

6 fold

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13
Q

What is the name of the clamp loader in eukaryotes?

A

Replication Factor C (RFC)

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14
Q

Shape of clamp loaders?

A

Pentameric

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15
Q

What is the name of the eukaryotic SSB?

A

Replication protein A (RPA1,2,3) [heterotrimer]

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16
Q

What is the name of eukaryotic primase?

A

Pol alpha

17
Q

What is the name of the prokaryotic primer?

A

DnaG

18
Q

Gene used to create telomeres?

A

hTERT

19
Q

Why is there telomerases?

A

RNA primer needs to be produced for the lagging strand?

20
Q

What happens to telomeres during ageing?

A

Shorten

21
Q

How does a cell know which strand is original?

A

Methylation

22
Q

How does proofreading work?

A

Incorrect nucleotides have a higher affinity for exonuclease active site