Initiation @ Termination Flashcards

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1
Q

What is the S value for prokaryotic ribosomes?

A

70S

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2
Q

What are the sub units of eukaryotic ribosomes?

A

40S and 60S

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3
Q

What are the sub units of prokaryotic ribosomes?

A

30S and 50S

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4
Q

Prokaryotic initiation code for ribosomes?

A

AGGAGG

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5
Q

What is the start code?

A

AUG

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6
Q

What does AUG code for in prokaryotes?

A

N-formyl-methionyl-tRNA

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7
Q

What does the P site hold?

A

tRNA with growing chain

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8
Q

What does the A site hold?

A

tRNA with new amino acid

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9
Q

What catalysed the exchange of the peptide chain to the new tRNA?

A

peptidyl transferase

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10
Q

What direction is the polypeptide chain synthesised?

A

N>C

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11
Q

What is wobble theory?

A

the anticodon of the tRNA in the A site can bind to multiple codons

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12
Q

What is the kozak sequence?

A

The sequence around AUG in eukaryotes which attracts ribosomes

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13
Q

What are the stop codons?

A

UAA UAG UGA

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14
Q

What do the stop codons code for?

A

Release factors

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15
Q

What does RF1 do?

A

recognises UAA UAG

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16
Q

What does RF2 do?

A

Recognised UAA UGA

17
Q

What does RF3 do?

A

Terminates RF1 and RF2