Producing New Cells Flashcards
In humans, there are 46 chromosomes, what is the number known as?
The chromosome compliment.
What has two matching sets of chromosomes?
Diploid cells.
What is important in maintaining the chromosome compliment?
Mitosis.
What is the first step in the process of mitosis?
Nucleus containing long uncoiled chromosomes each doubling to form 2 identical chromatids.
What is the second step in the process of Mitosis?
Short coiled up chromosome (made of two chromatids)
What is the third step in the process of Mitosis?
Chromosome attached at equator.
What is the fourth step in the process of Mitosis?
Pair of identical Chromatids separated and moving to opposite poles.
What is the fifth step in the process of Mitosis?
Separated chromatids now called chromosomes.
What is the sixth and final step in the process of Mitosis?
Two daughter cells each containing same number of chromosomes as original cell. Them cell growth prior to repeat of process.
What is Mitosis required for?
Growth and Repair.
Cells can be grown outside of their natural environment using?
Cell-culture techniques.
What can nutrient broth and nutrient agar be used for?
Growing Microbes.
Give 5 examples of aseptic techniques to be used when culturing cells?
Wear lab coat to protect clothes, wash hand before and after experiment to remove micro-organisms, clean work surface with disinfectant before and after experiment to kill microbes, have the dish sealed with tape to prevent any entry or exit of materials, lid held over Petri dish during inoculation to prevent entry of contaminations, wire loop flamed before use to kill unwanted microbes.
During cell culturing what should be monitored and controlled?
Oxygen Concentration, Temperature and PH.
