Principles of Clinical Chemistry

Question 1

Can be detected in a blood sample to confirm a Myocardial Infarction event?

Answer 1

Troponin I

Question 2

Define “plasma”

Answer 2

Blood with all cells removed

Question 3

Define “serum”

Answer 3

Blood with cells and clotting factors removed

Question 4

What is “Selective Requesting”? What are the two types?

Answer 4

Tests carried out on basis of patients clinical situation, divided into Core and Specialised

Question 5

What are the two types of Variation which can occur in biological samples

Answer 5

Analytical Variation

Biological Variation

Question 6

What is “Sensitivity” when discussing assays

Answer 6

How little of the analyte can a method detect

Question 7

What is “Specificity” when discussing assays

Answer 7

How good is the assay at discriminating between the required analyte and interfering substances

Question 8

Give examples of Liver Function Parameters

Answer 8

Bilirubin
AST and ALT
ALP
GGT
Plasma proteins
Prothrombin Time

Question 9

What is the half-life of Albumin?

Answer 9

20 days

Question 10

What does an elevated Bilirubin suggest?

Answer 10

Bile duct obstruction

Question 11

What does an elevated AST and ALT suggest?

Answer 11

General liver damage

Question 12

What does an elevated ALP suggest?

Answer 12

Cholestasis

Question 13

What does an elevated GGT suggest?

Answer 13

Cholestasis

Question 14

What enzyme regulates Phenyalanine in the blood?

Answer 14

Phenylalanine hydroxylase

Question 15

Give examples of ways laboratory specimens can be transported

Answer 15

Pneumatic tube system

Van transport