Principles Flashcards
Which part of an antibody do immune cells bind to?
Fc region
What is the most commonly produced immunoglobulin in the body?
IgA, however IgG is in the highest blood concentration.
What is the role of IgD?
Activation of B cells
What is the role of IgE?
Prevention against parasites and type 1 hypersensitity (allergies)
What is the role of IgG
Immune complex mediated rections - enhances phagocytosis
What is the role of IgM?
Produced in acute stages of infection and in cell bound type 2 hypersensitivity reactions.
What is the main source of IL-1?
Macrophages and monocytes
What type of medications may be used in an anaphylactic reaction?
Adrenaline, hydrocortisone, and chlorphenamine
What type of immune cells produce large abounds of specific antibodies?
Plasma cells
What is responsible for activating macrophages?
Interferon gamma
What triggers the mannose binding lectin pathway?
Mannose binding lectin binds to carbohydrates in the surface of pathogens.
What type of T cells mediate allergic asthma?
Th2 Cells
What type of immune cells is responsible for defence against protozoa and helminths?
Eosinophils
What is genetic nondisjuction?
Failure of the sister chromosomes to separate during mitosis. This can result in aneuploidy.
What gene is defective in Li-fraumeni syndrome?
p53
What is heteroplasmy?
The presence of more than one type of organellar genome - mitochondrial DNA or plastid DNA - within a cell or indicidual
What is genetic linkage?
Two genes that are located close to each other on a chromosome are often inherited together.
What is somatic hypermutation?
Where point mutation accumulate in the antibody V-regions of both heavy and light chains
What does a low Km value represent?
High affinity of an enzyme for its substrate
What is metaplasia?
Change from one mature cell type to another
What is neoplasia?
Abnormal cell proliferation
What is hyperplasia?
Physiological increase in cell numbers
What is hypoplasia?
Physiological decrease in cell numbers
What is morphogenesis?
The formation of a body plan during development
How long can ATP supply energy?
4 seconds
How long can phosphocreatinine supply energy?
15 seconds
How long can free circulating glucose supply energy?
4 minutes
How long can glycogen stores supply energy?
77 minutes
How long can fat stores supply energy?
4+ days
What component of MRSA causes a necrolytic skin infection?
Panton Valentine Leukocidin
What is Down’s syndrome?
Trisomy 21, patients have three copies of chromosome 21
What form of inheritance is Sickle Cell anaemia?
This is a form of co-dominant inheritance
What is Klienfelter syndrome?
47XXY, they have 3 sex chromosomes and therefore have 49 in total
How many transmembrane proteins make up G proteins?
7
What is an adenoma?
A benign tumour derived from glandular epithelium
What is a papilloma?
A benign tumour derived from mucous membrane
What does the suffix -carcinoma represent?
Malignant
What does the suffix -sarcoma represent?
Partially malignant, partially beinign
What does the suffix -oma represent?
Benign
How is CO2 transported in the body?
Dissolved in the blood
As carboxyhaemoglobin
As HCO3
As carbamino compounds
What is the most common method of CO2 transportation?
As HCO3
Describe Pseudomonas aeruginosa…
A gram negative coccabicallus. Resistant to most oral antibiotics
What is immunophenotyping?
Investigation of the pattern of antigen expression on or in cells through a panel of antibodies
What is light microscopy?
An techniques used for the morphological assessment of cells
What is alpha haemolysis?
Partial haemolysis - green colour
What is gamma haemolysis?
No haemolysis
What is beta haemolysis?
Complete haemolysis - yellow colour
Describe neisseria meningitidis…
Aerobic gram negative coccus
Where dies fertilisation normally occur?
Ampulla of the uterine tube
What is atrophy?
Degeneration of cells
What is the efficacy of a drug?
The ability of an agonist to evoke a cellular response
Why is there a decreased blood volume in patients with sepsis?
Endotoxins cause vasodilation and intravascular fluid to leak into adjacent tissue
How can we classify streptococci?
They are classified according to their haemolysis on blood agar
What are the four medical principles defined by Beauchamp and Childress?
Beneficence
Non-maleficence
Autonomy
Justice
What is an agonist?
This binds to the enzymes active site to enhance its effect
What is an antagonist?
This bind to the active site to block the binding of other molecules
What is FiFoATPase?
This is a proton pore which utilises the energy yielded from the return of protons along their electrochemical gradient in a condensation reaction with ADP and Pi to yield ATP
What activates C3 in the complement pathway?
The classical, alternative and lectin pathways
How do gram positive bacteria stain?
Purple due to thick peptidoglycan layer
How do gram negative bacteria stain?
Pink due to thin peptidoglycan layer
What is a granuloma?
A collection of macrophages that is a manifestation of an inflammatory process.
What is the role of ogliodendrocytes?
Produce myelin in the CNS
What receptor is responsible for glucose transport into the gut?
GLUT 5 - passive facilitated diffusion
Which G protein does M1 bind to?
Gq - stimulation of phospholipse C
What is the definition of Km?
The concentration of substrate which permits the enzyme to achieve half Vmax
What is muscle spasticity?
A muscle with increased tone, intact and functioning motor nerve but descending controls from the brain are not working
What is muscle paralysis?
Muscle with reduce tone, unable to contract and no functioning motor nerve supply
What is the rate limiting enzyme for the TCA cycle?
Isocitrate Dehydrogenase
What cells are the main producer of cytokines?
T helper cells
What is a type 1 hypersensitivity reaction?
Allergic Reaction
What is a type 2 hypersensitivity reaction?
Cytotoxic reaction
What is a type 3 hypersensitivity reaction?
Immunocomplex reaction
What is a type 4 hypersensitivity reaction?
Cell-mediated reaction
What causes the immune reaction in type 1 hypersensitivity?
IgE
What immune system components react to bacteria?
Antibodies and B lymphocytes
Phagocytes
What immune system components react to viruses?
T lymphocytes
Antibodies and B lymphocytes
What immune system components react to fungi?
T lymphocytes
Phagocytes
Complement proteins
What immune system components react to protozoa?
T lymphocytes
Eosinophils
What immune system components react to worms?
Eosinophils
Mast cells
What is inoculum size?
Median infecting dose required to cause disease in 50% of patients
What are the primary lymphoid tissues?
Thymus and Bone
Where the immune cells originate
What are the secondary lymphoid tissues?
Lymph Nodes, Adenoids, Tonsils, Spleen, Peyer’s Patches, Thoracic Duct
Where the immunity is propagated and refined
What are cytokines?
Small polypeptides released by a cell in order to change the function of the same or another cell
What is opsonisation?
The coating of pathogens by soluble factors (opsonins) to enhance phagocytosis
What are examples of opsonins?
C3b
CRP
IgG
IgM
What is the action of macrophages?
PAMPs bing to PRRs on the Mast Cell. This causes degranulation of inflammatory substances. There is then production of new pro-inflammatory mediators.
What are examples of pro-inflammatory mediators?
Leukotrienes Prostaglandins Nitric Oxide Histamines Pro-Inflammatory Cytokines (TNF alpha)
What are Leukotrienes?
Inflammatory chemicals the body releases after coming in contact with an allergen or allergy trigger.
What are Prostaglandins?
A group of lipids made at sites of tissue damage or infection that are involved in dealing with injury and illness. They control processes such as inflammation, blood flow, the formation of blood clots and the induction of labour.
What is the role of neutrophils?
They phagocytose and kill micro-organisms by releasing antimicrobial compounds
What do proinflammatory mediators promote?
Vascular changes
Recruitment and activation of neutrophils
What is diapedesis?
The passage of blood cells (e.g.neutrophils) through the walls of intact blood vessels
What is chemotaxis?
The movement of a compound in response to a stimulus
What are the three neutrophil killing mechanisms?
Phagocytosis
Degranulation
Neutrophil Extracellular Traps (NETs)
What are neutrophil extracellular traps?
The neutrophils expel the contents of their nuclei and granule contents. This releases digestive enzymes that can trap and kill pathogens
What is TNF alpha?
Tumour Necrosis Factor alpha is an inflammatory cytokine produced by macrophages/monocytes during acute inflammation and is responsible for a diverse range of signalling events within cells, leading to necrosis or apoptosis.
What is the difference between macrophages and monocytes?
Macrophages are monocytes that have migrated to the tissues.
What is the role of dendritic cells?
Activation if naieve T lymphocytes to initiate that adaptive immune response