Presumptive Tests (Exam 3)

Question 1

What are the 3 presumptive tests used to detect the presence of seminal fluid?

Answer 1

1) Acid phosphatase
2) Barberios crystal
3) Florence crystal

Question 2

How much more likely is acid phosphatase to appear in semen than in any other body fluid?

Answer 2

400x

Question 3

What is another name for acid phosphatase?

Answer 3

Prostate phosphatase

Question 4

What are the two products of the reaction of sodium alpha naphthyl phosphate and acid phosphatase?

Answer 4

1) Alpha-naphthol
2) Phosphate

Question 5

What is the name of the purple compound formed in the AP spot test after the addition of Diazo Blue?

Answer 5

Purple Diazonium

Question 6

What does PSA stand for?

Answer 6

Prostate Specific Antigen

Question 7

What do the “p” and “30” in p30 refer to?

Answer 7

P = glycoprotein found in prostatic epithelial cells
30 = 30kDa, or 30000 Daltons of molecular weight

Question 8

Define the King-Armstrong unit.

Answer 8

Amount of AP needed to hydrolyze 1mg of phenol from phenyl phosphate at 37.5 degrees C for 30 minutes at pH 9.1

Question 9

What 3 chemicals are needed to conduct the Florence crystal test?

Answer 9

Potassium iodide, iodine, di water

Question 10

How much KI, I, and diH2O are needed, respectively, for the Florence crystal test?

Answer 10

1.65g, 2.54g, and 30mL, respectively