Confirmatory Tests (Exam 2)

Question 1

What component of hemoglobin is tested with a crystal test?

Answer 1

Non-protein heme group

Question 2

What is hemoglobin?

Answer 2

O2-carrying protein of erythrocytes

Question 3

What class of compounds does hemoglobin belong to?

Answer 3

Porphyrins

Question 4

The chemical structure of heme relies on this atom with this bonding ability…

Answer 4

Hexavalent iron atom

Question 5

What are the 2 types of crystal tests?

Answer 5

Teichman & Takayama

Question 6

Teichman (1853) used these 4 components in his confirmatory test.

Answer 6

1) Bloodstain
2) Chloride
3) Acetic acid
4) Heat

Question 7

What are 2 different names for the Takayama crystal test?

Answer 7

1) Pyridine ferroprotoporphyrin
2) Hemochromogen

Question 8

What are the 4 components of the Takayama crystal test?

Answer 8

1) Pyridine (x1)
2) NaOH (x1)
3) Glucose (x1)
4) Water (x2)

Question 9

What key product is expected from the Takayama crystal test?

Answer 9

Pink, feather-like crystals