PRELIM LAB 2: PIPETTING Flashcards
PIPETTE CLASSIFICATION
I. DESIGN
II. DRAINAGE CHARACTERISTICS
III.TYPE
a. MEASURING OR GRADUATED
b. TRANSFER
PIPETTES ACCORDING TO DESIGN
TO CONTAIN (TC)
TO DELIVER (TD)
CALIBRATED TO CONTAIN A SPECIFIED AMOUNT OF LIQUID BUT ARE NOT NECESSARILY CALIBRATED TO DELIVER THAT EXACT AMOUNT
A TO-CONTAIN PIPETTES
B TO-DELIVER PIPETTES
A TO-CONTAIN PIPETTES
CALIBRATED TO DELIVER THE AMOUNT OF FLUID DESIGNATED ON THE PIPETTE
A TO-CONTAIN PIPETTES
B TO-DELIVER PIPETTES
B TO-DELIVER PIPETTES
VOLUME WILL FLOW OUT BY GRAVITY WHEN THE PIPETTE IS HELD VERTICALLY
A TO-CONTAIN PIPETTES
B TO-DELIVER PIPETTES
B TO-DELIVER PIPETTES
SMALL AMOUNT OF FLUID WILL CLING TO THE INSIDE WALL OF THE PIPETTE
A TO-CONTAIN PIPETTES
B TO-DELIVER PIPETTES
A TO-CONTAIN PIPETTES
PIPETTES ACCORDING TO DRAINAGE CHARACTERISTICS
BLOWOUT
SELF-DRAINING
HAS A CONTINUOUS ETCHED RING OR 2 SMALL,CLOSE, CONTINUOUS RINGS LOCATED NEAR THE TOP OF THE PIPETTE
A SELF-DRAINING
B BLOWOUT
B BLOWOUT
ALLOW THE CONTENTS OF PIPETTE TO DRAIN BY GRAVITY
A SELF-DRAINING
B BLOWOUT
A SELF-DRAINING
THE TIP OF THE PIPETTE SHOULD NOT BE IN CONTACT WITH THE ACCUMULATING FLUID IN THE RECEIVING VESSEL DURING DRAINAGE
A SELF-DRAINING
B BLOWOUT
A SELF-DRAINING
PIPETTES ACCORDING TO TYPE (MEASURING OR GRADUATED)
SEROLOGIC
MOHR
BACTERIOLOGIC
BALL, KOLMER, OR KAHN
MICROPIPETTE
PIPETTES ACCORDING TO TYPE (TRANSFER)
VOLUMETRIC
OSTWALD FOLIN
PASTEUR PIPETTES
AUTOMATIC MACRO PIPETTES/MICROPIPETTES
- IT HAS SEVERAL GRADUATION OR CALIBRATION MARKS
- USED PRIMARILY IN MEASURING REAGENTS
A MEASURING (GRADUATED) PIPETTES
B TRANSFER (VOLUMETRIC) PIPETTES
A MEASURING (GRADUATED) PIPETTES
- HAS BEEN CALIBRATED TO DELIVER A FIXED VOLUME OF LIQUID BY DRAINAGE
– USED TO MEASURE STANDARD SOLUTIONS, UNKNOWN BLOOD AND PLASMA, SERUM, PLASMA, URINE, CEREBROSPINAL FLUID, AND SOME REAGENTS
A MEASURING (GRADUATED) PIPETTES
B VOLUMETRIC (TRANSFER) PIPETTES
B VOLUMETRIC (TRANSFER) PIPETTES
- CONSIST OF CYLINDRICAL BULB JOINED AT BOTH ENDS TO NARROW GLASS TUBING
- CALIBRATION MARK IS ETCHED AROUND THE UPPER SUCTION TUBE, AND THE LOWER DELIVERY TUBE IS DRAWN OUT TO A FINE TIP
A MEASURING (GRADUATED) PIPETTES
B VOLUMETRIC (TRANSFER) PIPETTES
B VOLUMETRIC (TRANSFER) PIPETTES
- A STRAIGHT PIECE OF GLASS TUBING WITH A TAPERED END, AND GRADUATION MARKS ON THE STEM, SEPARATING IT INTO PARTS
A MEASURING (GRADUATED) PIPETTES
B VOLUMETRIC (TRANSFER) PIPETTES
A MEASURING (GRADUATED) PIPETTES
WHAT TYPE OF PIPETTE
- TD
- CALIBRATED BETWEEN 2 MARKS ON THE STEM (0-10)
- ORIFICE IS SMALLER
MOHR OR SEROLOGIC?
MOHR
WHAT TYPE OF PIPETTE
- TC
- GRADUATION MARKS UP TO THE FINE TIP (10-0)
- ORIFICE IS LARGER (DRAINS FASTER)
MOHR OR SEROLOGIC?
SEROLOGIC
MEASURING PIPETTE SPECIFICATIONS
Printed on the neck of the pipette are the specification that indicate:
- maximum volume of liquid that can be transferred
- size of the divisions of the pipette
- temp. at which calibrations were made
- TD and TC initials
ALL READINGS MUST BE MADE WITH THE ____ AT THE LEVEL OF THE MENISCUS
EYE
