PRELIM LAB 2: PIPETTING

Question 1

PIPETTE CLASSIFICATION

Answer 1

I. DESIGN
II. DRAINAGE CHARACTERISTICS
III.TYPE
a. MEASURING OR GRADUATED

       b. TRANSFER

Question 2

PIPETTES ACCORDING TO DESIGN

Answer 2

TO CONTAIN (TC)
TO DELIVER (TD)

Question 3

CALIBRATED TO CONTAIN A SPECIFIED AMOUNT OF LIQUID BUT ARE NOT NECESSARILY CALIBRATED TO DELIVER THAT EXACT AMOUNT

A TO-CONTAIN PIPETTES
B TO-DELIVER PIPETTES

Answer 3

A TO-CONTAIN PIPETTES

Question 4

CALIBRATED TO DELIVER THE AMOUNT OF FLUID DESIGNATED ON THE PIPETTE

A TO-CONTAIN PIPETTES
B TO-DELIVER PIPETTES

Answer 4

B TO-DELIVER PIPETTES

Question 5

VOLUME WILL FLOW OUT BY GRAVITY WHEN THE PIPETTE IS HELD VERTICALLY

A TO-CONTAIN PIPETTES
B TO-DELIVER PIPETTES

Answer 5

B TO-DELIVER PIPETTES

Question 6

SMALL AMOUNT OF FLUID WILL CLING TO THE INSIDE WALL OF THE PIPETTE

A TO-CONTAIN PIPETTES
B TO-DELIVER PIPETTES

Answer 6

A TO-CONTAIN PIPETTES

Question 7

PIPETTES ACCORDING TO DRAINAGE CHARACTERISTICS

Answer 7

BLOWOUT
SELF-DRAINING

Question 8

HAS A CONTINUOUS ETCHED RING OR 2 SMALL,CLOSE, CONTINUOUS RINGS LOCATED NEAR THE TOP OF THE PIPETTE

A SELF-DRAINING
B BLOWOUT

Answer 8

B BLOWOUT

Question 9

ALLOW THE CONTENTS OF PIPETTE TO DRAIN BY GRAVITY

A SELF-DRAINING
B BLOWOUT

Answer 9

A SELF-DRAINING

Question 10

THE TIP OF THE PIPETTE SHOULD NOT BE IN CONTACT WITH THE ACCUMULATING FLUID IN THE RECEIVING VESSEL DURING DRAINAGE

A SELF-DRAINING
B BLOWOUT

Answer 10

A SELF-DRAINING

Question 11

PIPETTES ACCORDING TO TYPE (MEASURING OR GRADUATED)

Answer 11

SEROLOGIC
MOHR
BACTERIOLOGIC
BALL, KOLMER, OR KAHN
MICROPIPETTE

Question 12

PIPETTES ACCORDING TO TYPE (TRANSFER)

Answer 12

VOLUMETRIC
OSTWALD FOLIN
PASTEUR PIPETTES
AUTOMATIC MACRO PIPETTES/MICROPIPETTES

Question 13

• IT HAS SEVERAL GRADUATION OR CALIBRATION MARKS
• USED PRIMARILY IN MEASURING REAGENTS

A MEASURING (GRADUATED) PIPETTES
B TRANSFER (VOLUMETRIC) PIPETTES

Answer 13

A MEASURING (GRADUATED) PIPETTES

Question 14

• HAS BEEN CALIBRATED TO DELIVER A FIXED VOLUME OF LIQUID BY DRAINAGE
  – USED TO MEASURE STANDARD SOLUTIONS, UNKNOWN BLOOD AND PLASMA, SERUM, PLASMA, URINE, CEREBROSPINAL FLUID, AND SOME REAGENTS

A MEASURING (GRADUATED) PIPETTES
B VOLUMETRIC (TRANSFER) PIPETTES

Answer 14

B VOLUMETRIC (TRANSFER) PIPETTES

Question 15

• CONSIST OF CYLINDRICAL BULB JOINED AT BOTH ENDS TO NARROW GLASS TUBING
• CALIBRATION MARK IS ETCHED AROUND THE UPPER SUCTION TUBE, AND THE LOWER DELIVERY TUBE IS DRAWN OUT TO A FINE TIP

A MEASURING (GRADUATED) PIPETTES
B VOLUMETRIC (TRANSFER) PIPETTES

Answer 15

B VOLUMETRIC (TRANSFER) PIPETTES

Question 16

• A STRAIGHT PIECE OF GLASS TUBING WITH A TAPERED END, AND GRADUATION MARKS ON THE STEM, SEPARATING IT INTO PARTS

A MEASURING (GRADUATED) PIPETTES
B VOLUMETRIC (TRANSFER) PIPETTES

Answer 16

A MEASURING (GRADUATED) PIPETTES

Question 17

WHAT TYPE OF PIPETTE
- TD
- CALIBRATED BETWEEN 2 MARKS ON THE STEM (0-10)
- ORIFICE IS SMALLER

MOHR OR SEROLOGIC?

Answer 17

MOHR

Question 18

WHAT TYPE OF PIPETTE
- TC
- GRADUATION MARKS UP TO THE FINE TIP (10-0)
- ORIFICE IS LARGER (DRAINS FASTER)

MOHR OR SEROLOGIC?

Answer 18

SEROLOGIC

Question 19

MEASURING PIPETTE SPECIFICATIONS

Printed on the neck of the pipette are the specification that indicate:

Answer 19

• maximum volume of liquid that can be transferred
• size of the divisions of the pipette
• temp. at which calibrations were made
• TD and TC initials

Question 20

ALL READINGS MUST BE MADE WITH THE ____ AT THE LEVEL OF THE MENISCUS

Answer 20

EYE