PRELIM LAB 1: LAB. SAFETY Flashcards
- It is the application of substance reaction and process in animals, plants, bacteria, virus.
- Also known as “Biological Chemistry” or “Chemical Biology”
BIOCHEMISTRY
When was Carl Alexander Neuberg introduced Biochemistry?
1903
What Enzyme is discovered by Carl Alexander Neuberg?
Hint: it incorporate CO2 in organic substances.
CARBOXYLASE ENZYME
What are the 2 Main BRANCHES of BIOCHEMISTRY?
Descriptive Biochemistry, Dynamic Biochemistry
BRANCHES OF BIOCHEMISTRY:
It focuses on the qualitative and quantitative study of chemistry in living beings.
A DESCRIPTIVE BIOCHEMISTRY
B DYNAMIC BIOCHEMISTRY
A DESCRIPTIVE BIOCHEMISTRY
BRANCHES OF BIOCHEMISTRY:
Study of chemical reactions that make life along with their mechanisms and nature.
A DESCRIPTIVE BIOCHEMISTRY
B DYNAMIC BIOCHEMISTRY
B DYNAMIC BIOCHEMISTRY
SAFETY IN BIOCHEMISTRY:
- can be set on fire on its own; can ignite on its own.
INFLAMMABLE
SAFETY IN BIOCHEMISTRY:
- can catch fire, can be set on fire
FLAMMABLE
SAFETY IN BIOCHEMISTRY:
- Used to control the heating (uniform heating).
OIL BATH
SCOPE OF BIOCHEMISTRY:
- Drug design and development
- Environmental Science
- Hospitals
- Forestry
- Agriculture
- Industry
- Dietetics
- Hormone production
- Vaccine Research
- Virology
- Immunology
- Microbiology
- Toxicology
- Food science
- Plant science
True or False
Never heat INFLAMMABLES solvents.
TRUE
use a ___________ as a heat source
OIL BATH, STEAM BATH, WATER BATH, HEATING MANTLE or HOT PLATE
ACCIDENTS IN BIOCHEMISTRY LABORATORY
*Chemical spills on skin
Wash area w/ running water for a minimum of ________
20 MINUTES
ACCIDENTS IN BIOCHEMISTRY LABORATORY
* ACID spillage
A Use BORIC ACID
B wash the area with a large volume of water for 20 mins.
C Use a solution of sodium bicarbonate (baking soda)
C Use a solution of sodium bicarbonate (baking soda)
ACCIDENTS IN BIOCHEMISTRY LABORATORY
* BASE spillage
A Use BORIC ACID
B wash the area with a large volume of water for 20 mins.
C Use a solution of sodium bicarbonate (baking soda)
A Use BORIC ACID
Ethyl ether and petroleum ether boiling point?
30-60 °C
The Henderson-Hasselbalch Equation:
pH = pKa + LOG (A-) / (HA)
ACID DISSOCIATION CONSTANT
Ka
NEGATIVE LOG OF Ka
pKa
CONJUGATE BASE
A-
WEAK ACID
HA
equilibrium concentration
NORMAL BLOOD pH:
7.35 - 7.45
ACIDOSIS:
< 7.34
ALKALOSIS:
> 7.44
HAZARDS IN THE BIOCHEMISTRY LABORATORY:
- adverse effects of a substance that result either from a single exposure or multiple exposures in a short space of time (usually less than 24 hours).
SEVERE TOXICITY
HAZARDS IN THE BIOCHEMISTRY LABORATORY:
- causes redness, inflammation
(reversible effects)
IRRITANT
HAZARDS IN THE BIOCHEMISTRY LABORATORY:
- “eat away” tissue gradually
(irreversible effects)
CORROSIVE
HAZARDS IN THE BIOCHEMISTRY LABORATORY:
- cause cancer
CARCINOGENIC
HAZARDS IN THE BIOCHEMISTRY LABORATORY:
- easily set on fire
FLAMMABLE
HAZARDS IN THE BIOCHEMISTRY LABORATORY:
- substances that pose a threat to
the health of living organisms, primarily that of humans.
BIOHAZARD
PERSONAL PROTECTIVE EQUIPMENT (PPE)
- Safety glasses
- Googles
- Apron
- Lab coats
- Gloves
- Protective shoes
- Respiratory protective equipment
- Ear defenders
