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BIOCHEM LAB > PRELIM LAB 1: LAB. SAFETY > Flashcards

PRELIM LAB 1: LAB. SAFETY Flashcards

1
Q
  • It is the application of substance reaction and process in animals, plants, bacteria, virus.
  • Also known as “Biological Chemistry” or “Chemical Biology”
A

BIOCHEMISTRY

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2
Q

When was Carl Alexander Neuberg introduced Biochemistry?

A

1903

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3
Q

What Enzyme is discovered by Carl Alexander Neuberg?

Hint: it incorporate CO2 in organic substances.

A

CARBOXYLASE ENZYME

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4
Q

What are the 2 Main BRANCHES of BIOCHEMISTRY?

A

Descriptive Biochemistry, Dynamic Biochemistry

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5
Q

BRANCHES OF BIOCHEMISTRY:

It focuses on the qualitative and quantitative study of chemistry in living beings.

A DESCRIPTIVE BIOCHEMISTRY
B DYNAMIC BIOCHEMISTRY

A

A DESCRIPTIVE BIOCHEMISTRY

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6
Q

BRANCHES OF BIOCHEMISTRY:

Study of chemical reactions that make life along with their mechanisms and nature.

A DESCRIPTIVE BIOCHEMISTRY
B DYNAMIC BIOCHEMISTRY

A

B DYNAMIC BIOCHEMISTRY

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7
Q

SAFETY IN BIOCHEMISTRY:
- can be set on fire on its own; can ignite on its own.

A

INFLAMMABLE

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8
Q

SAFETY IN BIOCHEMISTRY:
- can catch fire, can be set on fire

A

FLAMMABLE

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9
Q

SAFETY IN BIOCHEMISTRY:
- Used to control the heating (uniform heating).

A

OIL BATH

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10
Q

SCOPE OF BIOCHEMISTRY:

A
  • Drug design and development
  • Environmental Science
  • Hospitals
  • Forestry
  • Agriculture
  • Industry
  • Dietetics
  • Hormone production
  • Vaccine Research
  • Virology
  • Immunology
  • Microbiology
  • Toxicology
  • Food science
  • Plant science
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11
Q

True or False

Never heat INFLAMMABLES solvents.

A

TRUE

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12
Q

use a ___________ as a heat source

A

OIL BATH, STEAM BATH, WATER BATH, HEATING MANTLE or HOT PLATE

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13
Q

ACCIDENTS IN BIOCHEMISTRY LABORATORY
*Chemical spills on skin

Wash area w/ running water for a minimum of ________

A

20 MINUTES

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14
Q

ACCIDENTS IN BIOCHEMISTRY LABORATORY
* ACID spillage

A Use BORIC ACID
B wash the area with a large volume of water for 20 mins.
C Use a solution of sodium bicarbonate (baking soda)

A

C Use a solution of sodium bicarbonate (baking soda)

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15
Q

ACCIDENTS IN BIOCHEMISTRY LABORATORY
* BASE spillage

A Use BORIC ACID
B wash the area with a large volume of water for 20 mins.
C Use a solution of sodium bicarbonate (baking soda)

A

A Use BORIC ACID

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16
Q

Ethyl ether and petroleum ether boiling point?

A

30-60 °C

17
Q

The Henderson-Hasselbalch Equation:

A

pH = pKa + LOG (A-) / (HA)

18
Q

ACID DISSOCIATION CONSTANT

A

Ka

19
Q

NEGATIVE LOG OF Ka

A

pKa

20
Q

CONJUGATE BASE

A

A-

21
Q

WEAK ACID

A

HA

22
Q

equilibrium concentration

NORMAL BLOOD pH:

A

7.35 - 7.45

23
Q

ACIDOSIS:

A

< 7.34

24
Q

ALKALOSIS:

A

> 7.44

25
Q

HAZARDS IN THE BIOCHEMISTRY LABORATORY:
- adverse effects of a substance that result either from a single exposure or multiple exposures in a short space of time (usually less than 24 hours).

A

SEVERE TOXICITY

26
Q

HAZARDS IN THE BIOCHEMISTRY LABORATORY:
- causes redness, inflammation
(reversible effects)

A

IRRITANT

27
Q

HAZARDS IN THE BIOCHEMISTRY LABORATORY:
- “eat away” tissue gradually
(irreversible effects)

A

CORROSIVE

28
Q

HAZARDS IN THE BIOCHEMISTRY LABORATORY:
- cause cancer

A

CARCINOGENIC

29
Q

HAZARDS IN THE BIOCHEMISTRY LABORATORY:
- easily set on fire

A

FLAMMABLE

30
Q

HAZARDS IN THE BIOCHEMISTRY LABORATORY:
- substances that pose a threat to
the health of living organisms, primarily that of humans.

A

BIOHAZARD

31
Q

PERSONAL PROTECTIVE EQUIPMENT (PPE)

A
  1. Safety glasses
  2. Googles
  3. Apron
  4. Lab coats
  5. Gloves
  6. Protective shoes
  7. Respiratory protective equipment
  8. Ear defenders

BIOCHEM LAB (8 decks)

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