FINALS LAB 1: MICROSCOPE Flashcards
The simplest type of compound microscope
BRIGHTFIELD COMPOUND MICROSCOPE
refers to the fact that magnified objects appear as dark objects against a bright background.
BRIGHTFIELD
means that a specimen positioned properly on the stage of a microscope and illuminated by a light source will be magnified by a two-lens system
COMPOUND
THE PATH OF LIGHT
- ILLUMINATOR
- CONDENSER
- OBJECTIVE LENSES
- OCULAR LENS
THE PATH OF LIGHT
- also known as the LIGHT SOURCE
ILLUMINATOR
THE PATH OF LIGHT
- has lenses that DIRECT THE LIGHT RAYS through the specimen
CONDENSER
THE PATH OF LIGHT
- light rays then pass into the objective lenses, the LENSES CLOSEST TO THE SPECIMEN
OBJECTIVE LENSES
THE PATH OF LIGHT
- then the image of the specimen is MAGNIFIED AGAIN BY THE EYEPIECE
OCULAR LENS
oil used in oil immersion
CEDAR WOOD OIL
CEDAR WOOD OIL REFRACTIVE INDEX
1.516
The use of immersion oil improves the RESOLVING POWER OF THE LENSES
T OR F?
TRUE
if oil is not used with an oil immersion objective lens, the image becomes ________, WITH POOR RESOLUTION
FUZZY
PARTS OF THE MICROSCOPE
- houses the optical parts in the upper part of the microscope (STRUCTURAL COMPONENTS)
HEAD/BODY
PARTS OF THE MICROSCOPE
- connects to the basse and supports the microscope head
- also used to carry the microscope (STRUCTURAL COMPONENTS)
ARM
PARTS OF THE MICROSCOPE
- supports the microscope and houses the illuminator (STRUCTURAL COMPONENTS)
BASE
PARTS OF THE MICROSCOPE
- remagnifies the image formed by the objective lens;
- STANDARD MAGNIFYING POWER: 10x (OPTICAL COMPONENTS)
EYEPIECE/OCULAR LENS
PARTS OF THE MICROSCOPE
- holds the eyepiece in place above the objective (OPTICAL COMPONENTS)
EYEPIECE TUBE
PARTS OF THE MICROSCOPE
- primary lenses that magnify the specimen (OPTICAL COMPONENTS)
OBJECTIVE LENSES
PARTS OF THE MICROSCOPE
- a rotating turret that houses the objectives (OPTICAL COMPONENTS)
NOSEPIECE
PARTS OF THE MICROSCOPE
- A BIGGER WHEEL used to adjust the LPO in focusing
- used for INITIAL FOCUSING
COARSE ADJUSTMENT
PARTS OF THE MICROSCOPE
- a SMALLER WHEEL for FINAL FOCUSING of the specimen using HPO and OIO;
- used also to make the specimen MORE VIVID
FINE ADJUSTMENT KNOB
PARTS OF THE MICROSCOPE
- where the specimen/slide is placed for focusing
STAGE
PARTS OF THE MICROSCOPE
- used to hold the slide in place
STAGE CLIPS
PARTS OF THE MICROSCOPE
- light source of the microscope
ILLUMINATOR
PARTS OF THE MICROSCOPE
- used to COLLECT and FOCUS the light from the illuminator on the specimen
CONDENSER
PARTS OF THE MICROSCOPE
- CONTROLS the amount of light reaching the specimen
IRIS DIAPHRAGM
PARTS OF THE MICROSCOPE
- moves the condenser up or down to control the lighting focus on the specimen
CONDENSER FOCUS KNOB
OBJECTIVE LENSES:
MAGNIFICATION: 4X
BAND COLOR: RED
SCANNER
OBJECTIVE LENSES:
MAGNIFICATION: 10X
BAND COLOR: YELLOW
- usually forms the general outline or wide portion of the object
LOW POWER OBJECTIVE (LPO)
OBJECTIVE LENSES:
MAGNIFICATION: 40X
BAND COLOR: BLUE
- longer than the LPO and it forms a bigger image if the object is in focus
- in most cases, it is used to ENLARGE specimens that are too small under LPO
HIGH POWER OBJECTIVE (HPO)
OBJECTIVE LENSES:
MAGNIFICATION: 100X
BAND COLOR: WHITE
- HIGHEST DEGREE OF MAGNIFICATION
- used to examine STAINED SMEAR preparations of microorganisms using immersion oil as their medium.
OIL IMMERSION OBJECTIVE (OIO)
CALCULATING THE MAGNIFICATION
TOTAL MAGNIFICATION = magnifying power of the eyepiece (10x) x magnifying power of the objective used
