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BIOCHEM LAB > FINALS LAB 1: MICROSCOPE > Flashcards

FINALS LAB 1: MICROSCOPE Flashcards

1
Q

The simplest type of compound microscope

A

BRIGHTFIELD COMPOUND MICROSCOPE

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2
Q

refers to the fact that magnified objects appear as dark objects against a bright background.

A

BRIGHTFIELD

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3
Q

means that a specimen positioned properly on the stage of a microscope and illuminated by a light source will be magnified by a two-lens system

A

COMPOUND

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4
Q

THE PATH OF LIGHT

A
  1. ILLUMINATOR
  2. CONDENSER
  3. OBJECTIVE LENSES
  4. OCULAR LENS
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5
Q

THE PATH OF LIGHT
- also known as the LIGHT SOURCE

A

ILLUMINATOR

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6
Q

THE PATH OF LIGHT
- has lenses that DIRECT THE LIGHT RAYS through the specimen

A

CONDENSER

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7
Q

THE PATH OF LIGHT
- light rays then pass into the objective lenses, the LENSES CLOSEST TO THE SPECIMEN

A

OBJECTIVE LENSES

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8
Q

THE PATH OF LIGHT
- then the image of the specimen is MAGNIFIED AGAIN BY THE EYEPIECE

A

OCULAR LENS

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9
Q

oil used in oil immersion

A

CEDAR WOOD OIL

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10
Q

CEDAR WOOD OIL REFRACTIVE INDEX

A

1.516

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11
Q

The use of immersion oil improves the RESOLVING POWER OF THE LENSES

T OR F?

A

TRUE

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12
Q

if oil is not used with an oil immersion objective lens, the image becomes ________, WITH POOR RESOLUTION

A

FUZZY

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13
Q

PARTS OF THE MICROSCOPE
- houses the optical parts in the upper part of the microscope (STRUCTURAL COMPONENTS)

A

HEAD/BODY

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14
Q

PARTS OF THE MICROSCOPE
- connects to the basse and supports the microscope head
- also used to carry the microscope (STRUCTURAL COMPONENTS)

A

ARM

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15
Q

PARTS OF THE MICROSCOPE
- supports the microscope and houses the illuminator (STRUCTURAL COMPONENTS)

A

BASE

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16
Q

PARTS OF THE MICROSCOPE
- remagnifies the image formed by the objective lens;
- STANDARD MAGNIFYING POWER: 10x (OPTICAL COMPONENTS)

A

EYEPIECE/OCULAR LENS

17
Q

PARTS OF THE MICROSCOPE
- holds the eyepiece in place above the objective (OPTICAL COMPONENTS)

A

EYEPIECE TUBE

18
Q

PARTS OF THE MICROSCOPE
- primary lenses that magnify the specimen (OPTICAL COMPONENTS)

A

OBJECTIVE LENSES

19
Q

PARTS OF THE MICROSCOPE
- a rotating turret that houses the objectives (OPTICAL COMPONENTS)

A

NOSEPIECE

20
Q

PARTS OF THE MICROSCOPE
- A BIGGER WHEEL used to adjust the LPO in focusing
- used for INITIAL FOCUSING

A

COARSE ADJUSTMENT

21
Q

PARTS OF THE MICROSCOPE
- a SMALLER WHEEL for FINAL FOCUSING of the specimen using HPO and OIO;
- used also to make the specimen MORE VIVID

A

FINE ADJUSTMENT KNOB

22
Q

PARTS OF THE MICROSCOPE
- where the specimen/slide is placed for focusing

A

STAGE

23
Q

PARTS OF THE MICROSCOPE
- used to hold the slide in place

A

STAGE CLIPS

24
Q

PARTS OF THE MICROSCOPE
- light source of the microscope

A

ILLUMINATOR

25
Q

PARTS OF THE MICROSCOPE
- used to COLLECT and FOCUS the light from the illuminator on the specimen

A

CONDENSER

26
Q

PARTS OF THE MICROSCOPE
- CONTROLS the amount of light reaching the specimen

A

IRIS DIAPHRAGM

27
Q

PARTS OF THE MICROSCOPE
- moves the condenser up or down to control the lighting focus on the specimen

A

CONDENSER FOCUS KNOB

28
Q

OBJECTIVE LENSES:
MAGNIFICATION: 4X
BAND COLOR: RED

A

SCANNER

29
Q

OBJECTIVE LENSES:
MAGNIFICATION: 10X
BAND COLOR: YELLOW
- usually forms the general outline or wide portion of the object

A

LOW POWER OBJECTIVE (LPO)

30
Q

OBJECTIVE LENSES:
MAGNIFICATION: 40X
BAND COLOR: BLUE
- longer than the LPO and it forms a bigger image if the object is in focus
- in most cases, it is used to ENLARGE specimens that are too small under LPO

A

HIGH POWER OBJECTIVE (HPO)

31
Q

OBJECTIVE LENSES:
MAGNIFICATION: 100X
BAND COLOR: WHITE
- HIGHEST DEGREE OF MAGNIFICATION
- used to examine STAINED SMEAR preparations of microorganisms using immersion oil as their medium.

A

OIL IMMERSION OBJECTIVE (OIO)

32
Q

CALCULATING THE MAGNIFICATION

A

TOTAL MAGNIFICATION = magnifying power of the eyepiece (10x) x magnifying power of the objective used

BIOCHEM LAB (8 decks)

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