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CCP 4.1 - Pharmaceutical Biochemistry (Lab) > PRELIM 01 - Instrumentation > Flashcards

PRELIM 01 - Instrumentation Flashcards

1
Q

Measures the hydrogen ion concentration and the pH of an unknown solution

A

pH meter

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2
Q

Most modern pH meters also have a __________ which allows automatic temperature corrections since pH may vary relative to the temperature

A

Temperature probe

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3
Q

A pH meter is equipped with __________ and __________

A

Glass electrode, Reference electrode

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4
Q

An electrode that consists of a thin, pH sensitive glass membrane, the inside of which contains a solution with constant concentration of hydrogen ions

A

Glass electrode

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5
Q

Most common reference electrode

A

Calomel electrode

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6
Q

An electrode that incorporates a salt bridge filled with a saturated potassium chloride (KCl) solution

A

Calomel electrode

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7
Q

Is a method to measure how much chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solutions

A

Spectrophotometry

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8
Q

Instrument used in spectrophotometry

A

Spectrophotometer

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9
Q

A spectrophotometer that makes the sample absorb light in the visible and near ultraviolet region of the electromagnetic spectrum

A

Ultraviolet-Visible (UV-VIS) spectrophotometer

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10
Q

Are assays used to measure the amount of light transmitted through a sample at a given wavelength

A

Colorimetric assays

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11
Q

UV/Vis spectrophotometer - Ultraviolet range

A

185-400 nm

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12
Q

UV/Vis spectrophotometer - Visible range

A

400-700 nm

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13
Q

IR spectrophotometer - Infrared range

A

700-15,000 nm

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14
Q

7 essential parts of UV/Vis spectrophotometer

A

White light source, Monochromator, Sample compartment, Detector, Read out device, Tungsten lamp, Deuterium lamp

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15
Q

Disperses light into its component’s wavelengths

A

Monochromator

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16
Q

Holds the transparent tube or cell containing the sample

A

Sample compartment

17
Q

Measures the amount of light transmitted

A

Detector

18
Q

Device that displays the reading

A

Read out device

19
Q

Used as a light source in the visible region

A

Tungsten lamp

20
Q

Used as a light source in the ultraviolet region

A

Deuterium lamp

21
Q

The ratio of the intensity of the transmitted light to the incidence light

A

Transmittance

22
Q

Refers to the amount of light absorbed by a sample

A

Absorbance

23
Q

Law that is involved in the determination of the concentration of the absorbing species in the analyte solution

A

Beer-Lambert’s law

24
Q

Law that states that the intensity of transmitted light decreases exponentially as any one of these factors increases

A

Beer’s law

25
Q

Is a measure of how strongly a solution absorbs light in a particular wavelength

A

Molar extinction coefficient

26
Q

Process used to separate or concentrate materials suspended in a liquid medium using the effect of gravity on the particles in suspension

A

Centrifugation

27
Q

Fictitious force; peculiar to a particle moving on a circular path

A

Centrifugal force

28
Q

Device used to separate the particles in a solution according to their size, shape, density, or the viscosity of the medium and the rotor speed

A

Centrifuge

29
Q

Spectrophotometer that offers 5 nm spectral split and has a wavelength range of 200-1100 nm

A

GENESYS 5 spectrophotometer

30
Q

Spectrophotometer used to measure UV/Vis absorbance from 200 to 1000 nm on 96 to/or 384-well microcplates

A

Multiskan go microplate spectrophotometer

31
Q

Allows smaller sample volume (Microliter)

A

Microplate

32
Q

The Beer-Lambert’s law is most accurate between the absorbance of __________ to __________

A

0.05 to 0.70

33
Q

Using a multiskan go microplate spectrophotometer, one must incubate up to _____ and shake the microplate

A

45°C

34
Q

A spreadsheet like __________ may be used to generate a standard calibration curve

A

Microsoft Office Excel

35
Q

4 applications of UV/Vis spectrophotometer

A

Reaction kinetics, Quantitation, pKa determination, DNA structural information

36
Q

4 things to avoid on or inside the cuvette because it diminishes the accuracy of the reading

A

Formation of bubbles, Turbidity, Fingerprints, Condensation

CCP 4.1 - Pharmaceutical Biochemistry (Lab) (4 decks)

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