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CCP 4.1 - Pharmaceutical Biochemistry (Lab) > FINAL 02 - Qualitative Tests for Carbohydrates > Flashcards

FINAL 02 - Qualitative Tests for Carbohydrates Flashcards

1
Q

Molisch’s test (Test for)

A

General test for carbohydrates

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2
Q

Molisch’s test (Composition)

A

α-Naphthol

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3
Q

Molisch’s test (Positive result)

A

Purple/red ring

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4
Q

Molisch’s test (Principle)

A

Dehydration

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5
Q

Molisch’s test produces a derivative of furfural known as __________

A

5-hydroxymethylfurfural

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6
Q

Fehling’s test (Test for)

A

Reducing sugars

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7
Q

Fehling’s test A (Composition)

A

CuSO4.5H2O (Copper sulfate pentahydrate)

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8
Q

Fehling’s test B (Composition)

A

K Na Tartrate

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9
Q

Fehling’s test (Positive result)

A

Brick red ppt

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10
Q

Fehling’s test (Principle)

A

Reduction

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11
Q

Benedict’s test (Test for)

A

Reducing sugars

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12
Q

In Benedict’s test, __________ provides the alkaline conditions which are required for the redox reaction

A

Sodium carbonate

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13
Q

In Benedict’s test, __________ complexes with the copper (II) ions so that they do not deteriorate to copper (I) ions during storage

A

Sodium citrate

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14
Q

Benedict’s test (Composition)

A

NaOH + CuSO4

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15
Q

Benedict’s test (Positive resut)

A

Brick red ppt

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16
Q

Benedict’s test (Principle)

A

Reduction

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17
Q

Barfoed’s test (Test for)

A

Monosaccharides

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18
Q

In Barfoed’s test, monosaccharides usually react in about __________ to __________ minutes

A

1-2 minutes

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19
Q

In Barfoed’s test, disaccharides take a much longer time to react with the reagent, about __________ to ___________ minutes

A

7-12 minutes

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20
Q

Barfoed’s test (Composition)

A

Cupric acetate + Acetic acid

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21
Q

Barfoed’s test (Positive result)

A

Brick red ppt

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22
Q

Barfoed’s test (Principle)

A

Reduction

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23
Q

Tollen’s test (Test for)

A

Aldehydes

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24
Q

Tollen’s test (Composition)

A

Silver + Ammonia

25
Q

Tollen’s test (Positive result)

A

Silver mirror

26
Q

Tollen’s test (Principle)

A

Redox

27
Q

Picric acid test (Test for)

A

Reducing sugars

28
Q

Picric acid test (Composition)

A

Picric acid

29
Q

Picric acid test (Positive result)

A

Mahogany red

30
Q

Picric acid test (Principle)

A

Reduction

31
Q

In picric acid test, the reducing sugars react with picric acid, also chemically known as __________, to form red colored picramic acid

A

2,4,6-trinitrophenol (TNP)

32
Q

In picric acid test, the reducing sugars react with picric acid, also chemically known as 2,4,6-trinitrophenol (TNP), to form red colored __________

A

Picramic acid

33
Q

Nylander’s test (Test for)

A

Reducing sugars

34
Q

Nylander’s test (Composition)

A

Na K tartrate + K/NaOH + Bi

35
Q

Nylander’s test (Positive result)

A

Brown

36
Q

Nylander’s test (Principle)

A

Reduction

37
Q

Osazone/Kowarsky test (Test for)

A

Mannose

38
Q

Osazone/Kowarsky test (Composition)

A

Phenylhydrazine

39
Q

Osazone/Kowarsky test (Positive result)

A

Yellow ppt

40
Q

Osazone/Kowarsky test (Principle)

A

Reduction

41
Q

Seliwanoff’s test (Test for)

A

Ketones/Ketohexoses (Fructose)

42
Q

Seliwanoff’s test (Composition)

A

Resorcinol in conc. HCl

43
Q

Seliwanoff’s test (Positive result)

A

Red/Pink

44
Q

Seliwanoff’s test (Principle)

A

Dehydration

45
Q

Keller-Killiani test (Test for)

A

Deoxysugars (Cardiac glycosides)

46
Q

Keller-Killiani test (Composition)

A

Acetic acid, Fe chloride, Sulfuric acid

47
Q

Keller-Killiani test (Positive result)

A

Reddish brown

48
Q

Keller-Killiani test (Principle)

A

Hydrolysis

49
Q

Mucic acid test (Test for)

A

Galactose

50
Q

Mucic acid test (Composition)

A

Nitric acid

51
Q

Mucic acid test (Positive result)

A

Insoluble ppt

52
Q

Mucic acid test (Principle)

A

Oxidation

53
Q

Moore’s test (Test for)

A

Glucose

54
Q

Moore’s test (Composition)

A

Heat with alkali

55
Q

Moore’s test (Positive result)

A

Caramel

56
Q

Bial’s test (Test for)

A

Pentoses

57
Q

Bial’s test (Composition)

A

Resorcinol + Fe

58
Q

Bial’s test (Positive result)

A

Blue green

59
Q

Bial’s test (Principle)

A

Dehydration

CCP 4.1 - Pharmaceutical Biochemistry (Lab) (13 decks)

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