Pregnancy Diagnosis Flashcards
what are the 3 types of ways to determine pregnancy
hormone assays
rectal palpation
ultrasonography
hormone assays
what is radioimmunoassay
req radioactive hormones
radioactive hormone competes with same hormone in a blood sample
hormone assays
what is Enzyme-Linked immunosorbent assay (ELISA)
largely replaced the RIA
no radioisotopes are req
how does enzyme linked immunosorbent assay work
2 types of antibodies req
hormone antibody is added
hormone is added and it binds to the hormone antibody
enzyme antibody is added and it binds to the hormone hormone antibody complex
substrate is added to solution and reacts with the enzyme antibody to form color
why is ELISA becoming the standard for pregnancy diagnosis
involves blood sample
quick results
inexpensive
accurate
how do we determine pregnancy by rectal palpation
pliable enough to grasp the repro tract, thin enough to identify ovarian structure and strong enough to prevent injury to cow
what can ultrasonography do for determining pregnancy
early preg diagnosis
identification of twins
determination of ovarian structures present
fetal sex
fetal age