PRE TEST

Question 1

During an antibody optimization the intensity is optimal, but there is also nonspecific staining

Answer 1

Decrease antigen retrieval

Question 2

Tissue embedded on different panes can be prevented by

Answer 2

not allowing paraffin to solidify too fast

Question 3

Cause of compression for frozen tissue

Answer 3

warm blade

Question 4

Hindering IHC standardization

Answer 4

High number of manufacturers/ source of reagents

Question 5

In order to use two mouse antibodies for multistaining, which method must be used

Answer 5

Sequential

Question 6

When using the Fite procedure, Mycobacteria stain

Answer 6

Red

Question 7

Stringency is important during in situ hybridization because it

Answer 7

Affects the specificity of the probe

Question 8

In a -25C cryostat, sections of liver are shredding

Answer 8

Raise the temperature to -10

Question 9

The hemotoxylin of choice for acidic trichrome procedure is

Answer 9

Iron Hematoxylin

Question 10

When alcohol is added to formalin solution the result is

Answer 10

Faster fixation

Question 11

Which of the following could be a cause for pale nuclear staining on an HE slide

Answer 11

Overoxidized Hematoxylin

Question 12

Monoclonal antibodies show weak staining because

Answer 12

dependence on the presence of a specific epitope