PRE LAB EXPERMENT 6

Question 1

(?) is the clumping together of any particulate matter, forming a visible larger complex (?) resulting from the interaction between an insoluble antigen (?) and its corresponding antibody (?).

Answer 1

Agglutination

agglutinate

agglutinogen

agglutinin

Question 2

(?), on the other hand, is the dissolution or destruction of cells bearing non-self-antigens due to intravascular or extravascular mechanisms leading to an irreversible leakage of cell contents.

Answer 2

Lysis

Question 3

(?) are some of the observable means to detect the presence of antigen-antibody interactions.

Answer 3

Agglutination and lysis

Question 4

2 MOST COMMON SEROLOGIC REACTIONS

Answer 4

 Agglutination
 Lysis

Question 5

DEMONSTRATION OF AGGLUTINATION REACTIONS USING SLIDE METHOD
Divide into (?)
Label as (?)
And then, in NC or Negative control division, put a drop of (?)
After that, place a (?) in each of the division
Blood sample should be a (?)
Put one drop of the (?) on each division (A, B, NC)
After dropping the blood sample, mix the reagent and the (?)

Answer 5

3

a, b, nc (negative control)

NSS

blood sample

5% red cell suspension (RCS)

red cell suspension

blood sample

Question 6

Make sure that you make use of a different tip of the stirrer for the different portion, to prevent

Answer 6

contamination

Question 7

Observe the result

Answer 7

macroscopically and then microscopically as well

Question 8

Interpret the results within

Answer 8

2 minutes.

Question 9

Interpret it either as:

Answer 9

Positive
Negative

Question 10

no agglutination, no lyses

Answer 10

negative

Question 11

TUBE METHOD FOR AGGLUTINATION REACTION
Prepare (2) plain test tubes and label them as A, B, and NC
Put (?) drops of anti-A in tube A
Put (?) drops of anti-B in tube B
Put (?) dropsofNSSintubeNC
After putting the drops of reagent, add the (?) as the sample

Answer 11

3

2

2

2

5% red cell suspension

Question 12

Add (?) drop each RCS per tube

Answer 12

one

Question 13

Cover the tubes with (?) and then mix each tube gently

Answer 13

parafilm

Question 14

After mixing, centrifuge the tubes for

Answer 14

15 seconds at 3400 rpm

Question 15

After centrifugation,

Answer 15

gently dislodged

Question 16

caused the cells to gather at the bottom forming a cell button.

Answer 16

centrifugation

Question 17

In order to make sure it is a true agglutination,

Answer 17

gently shake/agitate the tubes

Question 18

• solid cell button (?).

Answer 18

positive result

Question 19

(the red cell antigen- antibody reaction serologic grading).

Answer 19

macroscopic evaluation

Question 20

(?), which has one solid aggregate with clear background

Answer 20

4+

Question 21

• cell button disappeared (?).

Answer 21

negative result

Question 22

No agglutination or hemolysis

Answer 22

negative result

Question 23

DEMONSTRATION OF HEMOLYSIS

Prepare (?) plain test tubes and label them as P (expected positive) and N (expected negative).
Place 2 drops of (?) in each tube.
Place 2 drops of (?) labeled as P.
Place 2 drops of (?) in tube labeled as N.
Cover and mix each test tube with (?).
Centrifuge for

Answer 23

two

5% red cell suspension

distilled water in tube

Normal Saline Solution (NSS)

Parafilm

15 seconds at 3,400 rpm

Question 24

T or F

(Do not dislodge the cell button. Whatever cell button is formed, you need to interpret it as it is).

Answer 24

T

Question 25

: intact cell button with clear supernatant

Answer 25

No hemolysis

Question 26

: presence of cell button with pink supernatant

Answer 26

Partial hemolysis (PH)

Question 27

: absence of cell button with red supernatant

Question 28

Test tube P have a little cell button (A [?] from the presence of cell button with pink supernatant). In test tube N, the result is the same with the test tube P.

Answer 28

partial hemolysis