PRE LAB EXPERIMENT 4

Question 1

Prepare two (2) test tubes and label one tube “0 – minute” and the others “5 – minute”. If there is enough blood available, a (?) can also be set up.

Answer 1

“10 – minute tube”

Question 2

Do a finger puncture and fill a heparinized microhematocrit tube about three-quarters full of blood. Note that blood drawn in an EDTA tube within the last (?) can also be used.

Answer 2

10 minutes

Question 3

Using a (?) (for capillary tube) or (?) (for whole blood anticoagulated with EDTA), expel one drop of blood into each labeled test tube.

Answer 3

black rubber bulb

Pasteur pipette

Question 4

Add (?) of Staphylococcus epidermis culture to each tube using a disposable Pasteur pipette. The culture should be no more than 0.5 McFarland standard in concentration. If necessary, dilute the standard in a broth tube or in sterile saline.

Answer 4

one drop

Question 5

Shake the tubes to mix, and make a blood film of the 0 tube immediately. Let the other tubes incubate for (?) and 10 minutes, respectively, at room temperature before making blood films from each of the sample mixtures.

Answer 5

5 minutes

Question 6

Obtain (?), one of which will be used as a spreader slide.

Answer 6

two clean glass slides

Question 7

With a (?), carefully place a small drop of blood at one end of a slide

Answer 7

Pasteur pipette

Question 8

Holding the spreader slide with the thumb and forefinger, place the spreader slide slightly in front of the drop of blood on the other slide, maintaining a (?) angle between the slides.

Answer 8

25°

Question 9

Move the (?) back toward the drop of blood. As soon as the slide comes in contact with the drop of blood, the blood will start to spread along the edge.

Answer 9

spreader slide

Question 10

Keeping the spreader slide at (?) angle, push it rapidly over the length of the slide. There should be a feathered edge on the end of the smear.

Answer 10

25°

Question 11

Note: These blood smears will be more watery than usual due to the addition of the (?); therefore, it may be well difficult to get a blood smear with a feathered edge. The feathered edge is not essential, as long as the blood cells are spread out on the slide.

Answer 11

broth culture with bacteria

Question 12

Allow the blood smear to (?).

Answer 12

air dry

Question 13

Stain according to typical (?) for staining blood smears.

Answer 13

laboratory protocol

Question 14

(?)the back of the slides to remove excess stain and let them air dry.

Answer 14

Blot

Question 15

Use (?) and look for engulfment.

Answer 15

immersion oil

Question 16

There should be a noticeable difference between the (?) slide.

Answer 16

0 – and the 5 – minute

Question 17

The (?) will probably not show much engulfment, but bacteria may be seen in contact with leukocytes.

Answer 17

0 – minute slide

Question 18

The (?) should show bacteria within the cell as small purple dots.

Answer 18

5 – minute slide

Question 19

(?) will be the predominant phagocytic cells, but an occasional (?) may be seen.

Answer 19

Neutrophils

monocyte

Question 20

If (?) are the only white blood cell seem, the bacterial suspension was too heavy, and the phagocytic cells destroyed themselves in attempting to engulf the bacteria present.

Answer 20

lymphocytes

Question 21

is the process by which specialized cells engulf and destroy pathogens (such as microorganisms) and damaged cells.

Answer 21

Phagocytosis

Question 22

are the most important phagocytic cells of the innate or natural immunity

Answer 22

Macrophages and segmented neutrophils (polymorphonuclears)

Question 23

A drop of (?) is mixed with a drop of a (?) and incubated at room temperature to demonstrate the engulfment of bacteria by leukocytes.

Answer 23

whole blood

bacterial culture

Question 24

This procedure may be useful in supporting the diagnosis of impaired (?) considering the presence of signs and symptoms

Answer 24

neutrophilic function