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IMMUNE1 LAB PRELIMS > PRE LAB EXPERIMENT 1 > Flashcards

PRE LAB EXPERIMENT 1 Flashcards

1
Q

Used to contain serum or other body fluids as well as the reagents in order for us to demonstrate a particular reaction.

A

PLAIN TEST TUBES

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2
Q

In serology laboratory, we are after

A

antigen- antibody reaction

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3
Q

Used to contain or transfer different volumes

A

SEROLOGICAL PIPETTES

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4
Q

NOTE: If we want to transfer a 5ml amount of fluid, we should use the pipette having a 5 ml amount of fluid; If we want to transfer 3 mL amount of fluid, it is much better to use (?) pipette instead of 10mL.

A

5 mL

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5
Q

To demonstrate agglutination or flocculation reactions

A

AGGLUTINATION CARDS/SLIDE (KLINE AGGLUTINATION SLIDE)

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6
Q

Example (flocuculationn reactions):

A

C-reactive protein Test (white- being the flocculation is white)
Antistreptolysin Test

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7
Q

specially in the preparation of red cell suspension, it is much better to use a

A

GRADUATED CENTRIFUGE TUBE

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8
Q

There are two types: glass and plastic

A

GRADUATED CENTRIFUGE TUBE

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9
Q

Used as a platform for microscopic specimen observation

A

GLASS SLIDE (ORDINARY)

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10
Q

Two types: glass and plastic

A

PASTEUR PIPETTE

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11
Q

Plastic if more preferred compared to glass

A

PASTEUR PIPETTE

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12
Q

: sensitive to glass Pasteur pipette

A

(Normal saline Solution)

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13
Q

Cloth and rubber

A

TOURNIQUET

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14
Q

used if the patient has a latex allergy or if a patient is hairy esp. in man

A

Cloth TOURNIQUET

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15
Q

disadvantage: the lock is easy to be broken

A

Cloth TOURNIQUET

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16
Q

Lavender:

A

EDTA

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17
Q

Blue:

A

sodium citrate

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18
Q

Gray:

A

sodium fluoride

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19
Q

Green:

A

heparin

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20
Q

: whether it does not contain an anticoagulant OR it contains a clot activator

A

Red

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21
Q

In drawing blood, the bevel upon inserting the needle should be facing you

A

Syringe

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22
Q

: always observe the hub if there is a backflow; pull the plunger slowly because if not, it is prone to hemolysis of the sample

A

Syringe

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23
Q

LANCET • Two types:

A

small lancet and feather lancet (ouch)

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24
Q

Has adjustments in order for you to choose what depth of puncture do you want

A

AUTOLANCET

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25
Q

Malfunctions most of the time

A

AUTOLANCET

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26
Q

NOTE: it is better to use (?) when it comes to capillary blood collection

A

manual force

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27
Q

Disadvantage: you can’t see the backflow of the hub especially if you have stick it already to the patient

A

2-WAY NEEDLE AND HOLDER/ADAPTER

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28
Q

Used to confirm sometimes the agglutination reaction

A

COMPOUND MICROSCOPE

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29
Q

Rotate to agglutination card/ slide to observe properly the agglutination reaction

A

MECHANICAL ROTATOR

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30
Q

For separating plasma and serum from the packed RBC

A

CLINICAL CENTRIFUGE

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31
Q

To demonstrate agglutination especially when using tube tests

A

CLINICAL CENTRIFUGE

32
Q

Use to sterilize glassware that are being used in serology laboratory

A

CLINICAL STERILIZER

33
Q

All sections in the lab are just sharing a(CC, CM, Serology)

A

1 clinical sterilizer

34
Q

Place some tests that require incubation

A

WATER BATH

35
Q

Same concept with clinical sterilizer

36
Q

Used to sterilize glassware

37
Q

1000 ul, 500 ul, 10-100 ul (yellow tip)

A

BLUNT TIP NEEDLE

38
Q

The tips will depend on the capacity of micropipette

A

BLUNT TIP NEEDLE

39
Q

used in serology laboratory

A

TUBERCULIN SYRINGE

40
Q

To administer allergen

A

TUBERCULIN SYRINGE

41
Q

For example: Mantoux test, For testing if you type IV hypersensitivity

A

TUBERCULIN SYRINGE

42
Q

used for immunoassays

A

MICROTITER PLATE

43
Q

For example: Enzyme immunoassays - this type of plate is already coated with a reagent

A

MICROTITER PLATE

44
Q

used to rim the sides of the test tube, also used for mixing

A

WOODEN APPLICATOR STICK/APPLICATOR STICK

45
Q

either with we put soap, most of the time in serology lab, we are placing here are your NSS (Normal saline solution), to be easier to make red cell suspension

A

WASH BOTTLE

46
Q

Part of analytical phase of quality assurance; process of monitoring results from control samples to verify accuracy of patient results.

A

QUALITY CONTROL (QC)

47
Q

Note: is not performed for your equipment but also each process of the laboratory. It is important to verify the accuracy of the patients result that we are releasing.

A

QUALITY CONTROL (QC)

48
Q

Sample that is chemically & physically similar to unknown specimen & is tested in exactly the same manner.

49
Q

Monitors precision of test system.

50
Q

For nonwaived quantitative tests, CLIA requires at least 2 levels of controls each day test is performed. (See “Equivalent QC” below.)

51
Q

For qualitative tests, pos & neg controls must be included with each run.

52
Q

Before we ran patient sample, make sure that we have ran first the control. Never ever release a sample if you did not yet run the control.

53
Q

Not only applicable in chemistry, but also all the section of the laboratory that will be using a machine.

54
Q

Testing control material not built into test system. Term also used for QC that extends beyond lab, e.g., participation in proficiency testing program.

A

EXTERNAL QC

55
Q

DOH mandated that all laboratory should participate in

A

national external quality assurance scheme (NEQAS).

56
Q

Electronic, Internal, or procedural controls that are built into test system.

A

INTERNAL MONITORING SYSTEMS

57
Q

This is what we practice inside the clinical laboratory.

A

INTERNAL MONITORING SYSTEMS

58
Q

Under that will also be the different QC will apply for each equipment that is being used.

A

INTERNAL MONITORING SYSTEMS

59
Q

Microscopes - Clean & adjust

A

4 times/yr.

60
Q

Temperatures - (?) of incubation, heating blocks, water baths, refrigerators, freezers.

A

Daily checks

61
Q

Quarterly

A

Speed
Timer

62
Q

Yearly

63
Q

Day of use

A

Tube fill level (serologic)

Temperature check (refrigerated centrifuge)

64
Q

Weekly

A

Saline fill volume (serologic)

65
Q

Volume of antihuman globulin dispensed (if applicable)

66
Q

Check temp daily

A

Waterbaths

Heat blocks

67
Q

Heat blocks

check each well.

A

Periodically

68
Q

Centrifuges

Determine optimum speed & time for different procedures

upon receipt -
after repairs -

A

QUARTERLY

PERIODICALLY

69
Q

Centrifuges

Check timer every

70
Q

Cell washers

Check tube fill level

71
Q

Cell washers

Verify time & speed.

72
Q

Pipettes Calibrate.

73
Q

Labs may reduce frequency of testing external OC materials for certain test systems if they can demonstrate through a CMS-approved QC evaluation process that test system is stable.

A

EQUIVALENT QC

74
Q

Here in the Philippines, we are not yet performing

A

EQUIVALENT QC

75
Q

Centrifuges

RPM every (with tachometer)

76
Q

Cell washers

AHG volume.

77
Q

Temperature verification (refrigerated centrifuge)

IMMUNE1 LAB PRELIMS (9 decks)

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