Practice #12 Flashcards
Systemic autoimmune diseases examples
SLE, RA, scleroderma, Sjogren’s syndrome
Organ specific autoimmune diseases examples
Myeasthenia gravis, Grave’s Basadow’s disease, Hashimoto’s , IDDM, Addison’s
Important antibodies to detect in autoimmune diseases
ANA, ANCA, ACPA, RF, anti phospholipid antibodies
Methods to detect in autoimmune diseases
ELISA, IF, Electrophoresis + WB
ANA
Anti nuclear autoantibodies
against: histones, dsDNA, topoisomerases, centromere, RNP
ACPA/ anti CCP
Anti cyclic citrullinated protein antibody
APS
Anti phospholipid syndrome (anti cell membrane proteins, prS, prC, prothrombin, cardiolipin, phosphatidyl serine)
RF
Against Fc fragment of IgG
ANCA
Anti neutrophil cytoplasmic antibodies
mainly against PR3 and MPO
but also elastase, lactoferrin, BPI
The classification of pANCA cANCA is based on
Formalin and ethanol fixation test
cANCA weakly cationic or neutral
pANCA strongly cationic
Positivity for ANCA can indicate
- Wegner granulomatosis
- PAN
- Churg- Strauss syndrome
Antiphospholipid anibodies detected by ELISA
Cardiolipin
Phosphatidylserine
beta2 glycoprotein I
Antiphospholipid anibodies detected by functional test
Prothrombin
Lupus anticoagulant
What type of Ig is RF?
IgM
Antibodies detected in SLE
ANA
anti dsDNA (can be detected 2.5 years before symptoms start)
anti Smith (can be detected few months before symptoms start)
anti chromatin
anti C1q
anti Ro (can be detected 10 years before symptoms start)
