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A Level Biology OCRA > Practicals > Flashcards

Practicals Flashcards

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1
Q

Iodine test: what is it a test for?

A

Starch

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2
Q

Iodine test: what is the method?

A

Add 1cm3 of sample to a dimple in spotting tile
Add a few drops of iodine in KL solution.

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3
Q

Iodine test: what is a positive result?

A

Blue/ black colour

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4
Q

Emulsion test: what does it test for?

A

Lipids

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5
Q

Emulsion test: what is the method?

A

Add 1cm3 of ethanol to a test tube
Add 1cm3 of sample
Shake vigorously to dissolve
Add 1cm3 of cold water

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6
Q

Emulsion test: what is the positive result?

A

Cloudy emulsion

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7
Q

Biuret test: what does it test for?

A

Protein

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8
Q

Biuret test: what is the method?

A

Add 2cm3 sample solution
Add 2cm3 of biuret solution
Observe colour change

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9
Q

Biuret test: what is a positive result?

A

Purple/ lilac colour.

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10
Q

Benedict’s test: what does it test for?

A

Reducing sugars

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11
Q

Benedict’s test: What is the method?

A

Add 2cm3 of sample solution
Add 2cm3 of Benedict’s reagent
Heat gently in water bath to 85 degrees for 2 minutes
Observe colour change

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12
Q

Benedict’s test: what is a positive result?

A

Green/ red colour
Brick red precipitate

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13
Q

Acid hydrolysis: what does it test for?

A

Non reducing sugars

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14
Q

Acid hydrolysis: what is an example of non reducing sugars?

A

Any sugar which gives a negative Benedict’s test

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15
Q

Acid hydrolysis: what is the method?

A

And 2cm3 of sample
Add 1cm3 of 1m HCL
Boil in water bath for 1 minute
Carefully neutralise with NaHCO3 powder
Check pH with indication paper

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16
Q

Acid hydrolysis: why do you have to carefully neutralise?

A

Effervescence will occur

17
Q

Acid hydrolysis: what is a positive result?

A

Green/red colour
Brick red precipitate

18
Q

How do you work out uncertainty?

A

Value of smallest division / 2

A Level Biology OCRA (7 decks)

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