Practical Modules 1-3 Flashcards

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1
Q

Primary Literature

A
  • The original publication of peer-reviewed research

- Usually written by scientists who carried out the research

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2
Q

Secondary Source

A
  • Another person’s interpretation of primary research

- Ex: critiques, summarizations of the findings in a review, or a report of said advances in an article

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3
Q

Scientific Hypothesis

A
  • A causal explanation of a natural phenomenon
  • Testable: can be tested, not hypothesis if it cannot be tested
  • Falsifiable: If not always true, can be proven false
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4
Q

Independent Variable and Dependent Variable

A
  • IV: Variables not changed by other variables
  • DV: Manipulated or measured between control and experimental group
    • Ex: Height= IV, Jumping distance= DV
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5
Q

Eukaryotic Cell

A
  • Have membrane-enclosed organelles
  • Nucleus
  • Both have plasma membrane and genetic material
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6
Q

Prokaryotic Cell

A
  • Do not have membrane-enclosed organelles
  • No nucleus, has DNA though
    • Replicate as quickly as they can
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7
Q

What are the two main fibers found in the extracellular matrix of areolar connective tissue? What are their functions?

A
  • Collagen fibers (large white fibers)
  • Elastic fibers (smaller, dark fibers)
    • Create stretch
  • Both make up the extracellular matrix
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8
Q

Cytoplasmic Streaming

A
  • The moving of chloroplasts in a plant cell in response to light
  • Acess to light= photosynthesis (create energy and O2
  • Chloroplasts moved in elodea leaf
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9
Q

Ocular Lens

A
  • Nearest to your eye when you are looking through the microscope at a specimen
  • 1 ocular= monocular, 2=binocular
  • Can be adjusted using interpupillary adjustment (pulling them apart or pushing them together)
  • 10X magnification
  • Pointer= black line that points at specimen
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10
Q

Objective Lens

A
  • Closest to the specimen
  • 3 or 4 arranged on a rotating nose piece
    • Rotating leads to different lens of diff magnification on specimen
    • 4x. 10x, 43x, 100x
  • Total mag= ocular x objective magnification
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11
Q

Body Tube

A
  • Part of microscope between oculars and objectives

- Light travels through specimen, through objectives, through tube, and to your eyes through ocular lens

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12
Q

Mechanical stage

A
  • the platform on which the slide is placed
  • Moved by means of two small knobs under the stage
    • One moves it left and right, the other moves it up and down
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13
Q

Substage

A
  • Area under the stage
  • Iris diaphragm: opens and closes like an eye to regulate amount of light that hits specimen (move level back and forth)
  • Condenser: Focuses light by a knob that moves it up and down
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14
Q

Base

A
  • The bottom of the microscope that sits on table
  • Holds the illuminator (light source)
    • Intensity can be changed using dial on the left side of base (1-10)–> set at 1 before turning it on
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15
Q

Arm

A
  • Connects the body tube to the stage and bade
  • Focusing knobs:
    • Larger: coarse focus– find specimen
    • Smaller: fine focus–bring object into focus
  • At greater than 10x objective, always use fine focus instead of coarse (don’t want to hit slide)
  • Move out of mag if can’t find it and use coarse
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16
Q

Field of view and depth of field

A
  • Field of view: Horizontal plane visible at certain magnification
  • Depth of field: Vertical direction in focus at one time
  • As magnification becomes higher, field of view and depth of view decrease
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17
Q

What type of cells line the inner passageways and outer coverings of your body?

A

-Epithelial cells

18
Q

What type of stain did we use to stain cheek cells? What structures of a cell is it good at staining?

A
  • Methylene Blue

- Good at staining the nucleus, cytoplasm, and cell membrane

19
Q

In vital staining, what types of cells or tissues are being stained?

A

-Live cells or tissues

20
Q

What type of organism is the gene for green fluorescent protein derived from?

A

-A jellyfish

21
Q

What is the function of the guard cells?

A
  • Change their shape to open and close the stomata

- Surrounds stomata

22
Q

What substances move through the stomata when they are open?

A

-Allow for gas exchange and water evaporation

23
Q

How do crypts and trichomes function in reducing water loss from plant leaves?

A
  • Crypts: help prevent water loss when the stomata are open by creating a humid environment (evaporation not as great)
  • Trichomes: hairs in crypt, slow evaporation
24
Q

Why do plants have the ability to close stomata via the guard cells?

A

-To prevent water loss

25
Q

What specializations do water lilies have for living in an aquatic environment?

A
  • Stomata in upper epidermis to reach air for gas exchange

- More spongy mesophyll: stay buoyant

26
Q

Cellular Respiration

A
  • Function: converts glucose and oxygen to carbon dioxide, water, and ATP (energy)
  • Occurs in mitochondria
  • Relates to gas exchange at cellular and organism level because O2 is exchanged with CO2
27
Q

Photosynthesis

A
  • Function: Carbon dioxide, water, and light energy–> Oxygen, glucose
  • Carried out by chloroplasts
  • Reaction: light reactions and Calvin cycle
  • Relates to gas exchange at the cellular and organismal level because light reactions produce O2 and Calvin cycle uses CO2 to create energy
28
Q

Light Reactions

A
  • Capture energy from sunlight

- Water is split to release electrons, producing O2

29
Q

Calvin Cycle

A

-CO2 and energy-containing compounds from the light reactions are used to create glucose

30
Q

BioChamber Experiment

A
  • In a light environment, the spinach leaves will perform photosynthesis, making the CO2 levels in the environment decrease (Calvin Cycle) and O2 levels will increase (light reactions)
  • In the dark environment, the CO2 levels will increase due to cellular respiration
31
Q

Basic Components of Scatterplot and Bar graphs

A
  • X axis (independent variable)
  • Y axis (dependent variable)
    • Proper units on bother axes
  • BG: average values for certain parts of x-axis
    • Ex: average height for different genders
  • NO CHART TITLE– figure caption instead*
  • SP: displays trends using individual points
    • Trendline (y=mx+b equation) and r^2 value (strength of relationship)
32
Q

Types of graphs

A
  • Pie chart (parts of a whole)
  • Bar graph (averages)
    • Qualitative data (categorical),
  • Line graph (changes over time)
  • Scatter plot (individual points)
    • Quantitative pieces of data
33
Q

What role do predictions play in designing experiments?

A
  • Follow up of the hypothesis
  • Guides the design of experiments or observational studies
  • Predictions are tested
34
Q

Calibrating Ocular Micrometer

A
  • Each line of ocular micrometer is 10 ocular units
  • Line it up with mm
  • mm/ocular units= calibration
  • Ex: .5mm= 50 ocular units–> 1 ocular unit= .01 mm
35
Q

What is the difference between mammalian erythrocytes and erythrocytes of other vertebrates? What is their primary function?

A
  • Mammalian RBCs have no nucleus, other vertebrates have nucleus in RBCs
    • No nucleus= more hemoglobin to transport more oxygen
  • Function= to transport oxygen throughout body
36
Q

How do Volvox and Scendedesmus obtain energy, and with what structure do they do so?

A

-Obtain energy from sun in their chloroplasts (photosynthesis)

37
Q

Scenendesmus

A
  • Unicellular
  • Lives in colonies
  • Non-motile (doesn’t move)
38
Q

Chlamydomonas

A

-Has flagella–> moves

39
Q

Volvox

A
  • Multicellular
  • Daughter cells= dots inside cell, darker
    • Reproduce asexually through cell division
  • Somatic cells= incapable of reproduction, bigger cell
  • Cilia= helps it move
40
Q

Human Blood Smear

A
  • Fragment/ little dots: platelets
  • Dark spots with circles inside: white blood cells, have nucleus
  • Lighter: red blood cells, no nucleus