Practical l (Photosynthesis) Flashcards
Cellular Respiration Equation
C6H12O6 (Glucose) + 6O2 –> 6CO2 + 6H20 +ATP
Photosynthesis Equation
6CO2 + 6H2O -(light energy)-> C6H12O6(Glucose) + 6O2
Where is Photosynthesis?
Chloroplast
Where is Cellular Respiration?
Mitochondria
Where does light reaction occur?
Thylakoid membrane
Where does Calvin Cycle occurs?
Stroma
What is Spectrophotometer use for ?
Measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution
What is Cuvette?
A special square tube
What is the Beer-Lamber Law?
A(Absorbance)=ε(coeffiecient)L(lenght)c(concentration)
Rate of Oxygen Synthesis in Elodea Procedure
Release of oxygen from a plant indicates that the light reactions of photosynthesis are occurring. Some of the oxygen released during photosynthesis is used by the plant when cellular respiration occurs. When using a green light, the rate of photosynthesis decreases
Carbon Dioxide Fixation Procedure
During the Calvin cycle reactions of photosynthesis, the plant takes up carbon dioxide and uses it to
produce carbohydrates, such as glucose (C6H12O6). Therefore, the carbon dioxide in the solution surround
Elodea should disappear as photosynthesis takes place. Free CO2 will combine with water to produce
carbonic acid.
Reducing Power of Electron Transport Procedure
In plants the electron acceptor is NADP+, but in the following experiment you will be using an artificial
electron acceptor 2,6-dichlorophenol-indophenol (DPIP). DPIP is blue in its oxidized form but turns
colorless in its reduced form. As the Hill reaction proceeds the reduction of DPIP will depend on the
rate of electron flow. By measuring the change in color of DPIP we can indirectly measure the electron flow.
This reaction requires a pH of 6.5, which is maintained by a phosphate buffer.
