Practical l (Cellular Respiration)

Question 1

What is a Aerobic Cellular Respiration?

Answer 1

Requires Oxygen
C6H12O6 (glucose) + 6O2 –> 6CO2 +6H2O +32ATP

Question 2

Citric Cycle (Kreb Cycle)

Answer 2

In eukaryotes, glucose breakdown begins in the cytoplasm, but is completed in mitochondria during the
Citric Acid Cycle. Electrons stored in NADH and FADH2 during the metabolism of glucose to CO2 feed
into the process of oxidative phosphorylation. During oxidative phosphorylation electrons move through
an electron transport chain in the inner mitochondrial membrane. This results in a build up of protons in the
intermembrane space of the mitochondria. The flow of protons down their concentration gradient through
ATP synthase leads to a large production of ATP in a process call chemiosmosis. Cellular respiration is
an aerobic process requiring oxygen. Oxygen is the final electron acceptor at the end of the electron
transport chain, which accepts protons to form water.

Question 3

What is a Fermentation (Anaerobic)?

Answer 3

It takes place inside the cytoplasm and does not require oxygen. ATP-generating process that involves the breakdown of glucose to either ethanol or
lactate.

Question 4

Ethanol Fermentation Formula

Answer 4

C6H12O6 (Glucose) –> 2CH3CH2OH (ethanol) + 2CO2 + 2ATP

Question 5

Lactic Acid Fermentation Formula

Answer 5

C6H12O6 (Glucose) –> 2CH3CHOHCOOH (lactate) + 2ATP

Question 6

Cellular Respiration- Production fo CO2 Procedure

Answer 6

Use yeast to find CO2. Use a Respirometer to measure gas volume changes