Practical l (Enzymes)

Question 1

Amylase

Answer 1

A digestive enzyme breaks down starch into maltose and glucose. To determine use Iodine test

Question 2

Pepsin

Answer 2

A digestive protease that breaks protein down into peptides and amino acids (ex: Milk)

Question 3

Catalase

Answer 3

Speeds the breakdown of hydrogen peroxide into water and oxygen. In the experiment, it shows that forming bubbles and heat

Question 4

Urease

Answer 4

Produces ammonia and CO2. The experiment used pH test strap 9 acidic) and sniff the tube to determine if it smells like nitrate

Question 5

Lysozyme

Answer 5

Breakdown bacterial cell walls, by catalyzing the hydrolysis of proteoglycans between NAM and NAG residues.Producing Proteoglycan pieces and clearing turbid solution

Question 6

Acid Phosphate

Answer 6

A hydrolytic enzyme that removes phosphate groups producing p-nitrophenol and acidic and yellow solution

Question 7

Temperature Effect on Amylase Activity

Answer 7

As temperature increases, amylase activity generally increases until reaching an optimal temperature, after which the activity rapidly declines due to the enzyme denaturing at high temperatures

Question 8

pH Effect on Amylase Activity

Answer 8

The optimal pH is 7 and decreasing or increasing of pH can increase or decrease the enzyme activity