Practical

Question 1

Beer Lambert Law

Answer 1

A = eM x c x l

Remember to convert from mol/L to mol/ml, and then mol/min to mol/sec for katals

Question 2

Michaelis menten

Answer 2

V0 = (Vmax [S]) / (Km + [S])

Lineweaver Burke plot:
1/V0 = (Km/Vmax) (1/[S]) + (1/Vmax)
x-intercept = -1/Km
y-intercept = 1/Vmax
gradient = Km/Vmax

Question 3

Non-polar amino acids (10)

Answer 3

Alanine, Valine, Leucine, Isoleucine, Glycine, Cysteine, Phenylalanine, Tryptophan, Methionine, Proline

Question 4

Negatively charged amino acids

Answer 4

Aspartic acid, Glutamic acid

Question 5

Positively charged amino acids

Answer 5

Lysine, Arginine, Histidine

Question 6

Uncharged polar amino acids

Answer 6

Serine, Threonine, Tyrosine, Asparagine, Glutamine

Question 7

Southern blotting

Answer 7

DNA transferred to a +ve charged nylon membrane. DNA attaches to membrane. Wash membrane to remove unbound probes. Labeled DNA will show if present

Western blot = protein
Northern blot = RNA

Question 8

PCR

Answer 8

Denaturing - 90 C - dsDNA to ssDNA
Annealing of primers - 50 C
Extension of new DNA - 72 C - by Taq polymerase