Practical Flashcards
In direct agglutination, the Abs should be __&__
IgM
IgG
In direct agglutination, the Ag should
Insoluble (particulate)
What case prozone phenomenon in brucella
Hight Ab ratio in relation to Ag with case no cross link
Definition of prozone phenomenon
Absence of agglutination in tubes because(Dr used the word “with”)of high concentration of Abs
How to overcome prozone phenomenon
1/ use more than 6 tubes
Dr: use wide range of dilution
2/ add coomb’s reagent (antihuman globulin )
What is the importance( بمعنى resalt) of prozone phenomenon
False -ve result
Name the steps of coomb’s reagent (antihuman globulin)
1/ lgG or lgM antibodies is injected to animal
2/ the animal immune system will secrets anti IgG antibody
3/ these anti IgG Abs will bind to Fc of IgG
4/ this binding will form cross linking Ag Ab complex
And congratulations, we can see visible agglutintion
Name the application that we use passive : quantitative
Antistreptolysin o (ASO) test
Name the application in which reversed passive or qualitative is used
Detection of lancefield groups of beta hemolytic streptococci
In passive ( quantitative ) the unknown is
Abs
In reversed passive (qualitative) the unknown is
Ag
In reversed passive latex is associated ( هذه من عندي ) with
Abs
In passive (quantitative) latex is coated with
Ag
Name a famous application of reversed passive
Pregnancy test
In passive and reversed passive the kind of Ag is
Soluble
Name the 2 inert particles
Latex and RBCs
Protein ___ can coat with any molecule and this protein is carried on or associated with _____
A
Staphylococci
Staph coagglutination test is similar to
Reversed passive
RBC coated with solube Ag to detect Abs , this method is called
Passive haemagglutination test
RBC coated with Abs to detect solube Ags , this method is called
Reversed passive hemagglutination
Haemagglutination is used mainly to
Diagnose viral infections
Answer below
Indirect coombs test
Uses
Method
+ve resalt
Detect free Rh Abs in serum of maternal
1/ blood sample from mother
2/ add Rh +ve RBCs and coombs reagent
Rh Abs coat Rh+ve RBCs –> agglutination by coombs reagent
Answer below
Direct coombs test
Uses
Method
+ve result
Detect Rh Abs coating RBCs in serum of newborn
1/ blood sample from newborn
2/ add coombs reagent
RBCs coated with Rh Abs –> agglutination by coobs reagent
Name another use of direct coombs test
Autoimmune hemolytic anemia
Neutralization reaction is used in
Diagnosis of certain viral infection
Precipitation line is shown on ___
Toxigenic corynebacterium or C diphtheria
Name the application of double immunodiffusion
Detect ability of corynebacterium or C diphtheria to produce toxin
Application of single immunodiffusion (quantitative)
Measure con. Of serum protein
Like complement and Ig isotypes
In single radial immunodiffusion (quantitative) the known con. is _____ and the unknown con. is ____
Ab (antiserum)
لانه ضد سيريم دم المريض الذي على
complement and Ig isotypes
Ag , which is the serum protein
In single radial immunodiffusion the diffused is
Ag forming precipitation ring
Immunoelectrophoresis is qualitative/quantitative
Qualitative
What is electrophoresis ?
Is the migration of solutes or particles like IgM in an electrical field
In immunoelectrophoresis the ____ the protein the ____ the distance it will travel
And vice versa
Larger
Shorter
Name the procedure of immunoelectrophoresis
1/ a serum sample is placed in a well in agar gel on glass slide than a current is passed through the ager
2/ protein ( Ag) moves in electric field according to charge and size
3/ A trough is cut into the agar and filled with Ab (?)
4/ the Ag and Ab diffuse together which cause “arc of precipitate
The serum sample placed on a glass slide contain
Mixture of protein like IgM and IgG
Name the application of electrophoresis
1/ diagnosis of immunodeficiency diseases
Detection of low amounts of one or more Ig isotypes عندما يصبح Ab هوAg :(
2/ testing the presence , absence, or unusual patterns of serum protein ( Ag)
E.x = human myeloma protein
Arc of precipitation occur in which ingel test
Electrophoresis
What is the method of ring precipitation?
Known Ab is introduced into small test + unknown Ag is added
Name the result of ring precipitation and name the point
Ring of precipitation
The point I called interface
What is the full form of ELISA
Enzymes linked immuno sorbent assay
ELISA is used to detect both Ab and Ag in a
Sample
Name the application of ELISA
diagnosis of viral infection
Diagnosis of bacteria infection
Diagnosis of drugs in blood and tissues
