PPT 6

Question 1

Immortality in cancer

Answer 1

• Normal cells can only replicate a certain amount of times > daarna apoptosis
  > voor oncogenic transformation moet de cell door deze barriere

Question 2

Serial passaging is used to

Answer 2

determine the amount of times a cell divides

Question 3

Number of replicative doublings depends on

Answer 3

• The species from which the cells were prepared
• Tissue of origin
• Age of the donor organism

Question 4

Embryonic stem (ES) cells can replicate unlimited when

Answer 4

provided with proper nutrients and are immortal

Question 5

Limit the number of division

Answer 5

• accumulation of physical stress
• Recording of number of cell divisions

Question 6

Proliferation of human cells limited by

Answer 6

Telemore length
> Telemores prevent end-to-end fusion of chormosmal DNA

Question 7

Geen telomeer?

Answer 7

looks like DSB > DNA repair

Question 8

Replication of telomeres causes …

Answer 8

Shortening
> doordat de RNA primer voor lagging strand niet kan binden, omdat het einde te kort is.
> Exonucleases within cells slowly chew on the ends

Question 9

Cancer can escape crisis by

Answer 9

Expressing telomerase
Extends telomere length
= a revers transcriptase that contains its own RNA primer
Heel actief tijdens embryogenese

Question 10

Maintain telnomeric length without telomerase

Answer 10

ALT
Interchromosmal copying mechanism
Polymerases replicating DNA on one chromosome may use sequences from a second chromosome as a template, before switching back