POB Exam 2 Flashcards
Photosynthesis does what
Convert solar energy into chemical energy of a carbohydrate
Carbon dioxide enters the leaves through
Stomata
Filled interior of chloroplast is called
Stroma
Flattened sacs in chloroplast is what
Thylakoids
Thylakoids are stacked to form what
Grana
Chlorophyll and other pigments part of the thylakoid membrane do what
Absorb solar energy
Chloroplast has a what kind of membrane
A double membrane
Outer and inner membrane
Photosynthetic equation
6CO2 + 6H20 —
What are the two set of reactions in photosynthesis
Light reactions (dependent of light)
Calvin cycle (independent of light)
Electromagnetic spectrum
Gamma ray—
What are the photosynthetic pigments
Chlorophyll a and b
Carotenoids
Each of them absorb a specific wavelength
Leaves appear green because what light is reflected
Green
All other colors absorbed, reason we see a color is because it is reflected off
Light reaction of photosynthesis contains what process
Photosystem 2
Electron transport chain
Photosystem 1
Where does the light reactions take place
Thylakoid membrane
Ps2 does what?
Pigment complex absorbs solar energy
—>
Energy passes from on pigment to
another until reaching reaction center —>
Electrons energized
(Electron acceptor)
Water is split to replace energized electrons —
At the electron transport chain what happens
Carries energy from ps2 to ps1
Energy is release to pump hydrogen ions into the thylakoid space forming a gradient
When the ions flow through atp synthase it forms ATP
PS1 does what
Absorbs solar energy
Low energy ps2 electrons used to replace those lost in ps1
Adjacent to enzyme that reduces NADP+ to NADPH
(Accept electrons from ps2 to do this)
Hoover dam = light reaction
Reservoir = thylakoid space bc it store something
Rain water (light from sun)
Hills that collect water (Ps2 and ps1)
Water kept inside reservoir - hydrogen ions
Water released that power turbine (atp synthase) energy is formed (atp)
Where do plants get their carbon to grow their tissue
Inorganic carbon through leaves
Calvin cycle purpose is to do what
Use co2 to produce carbohydrate for plant
Where does the Calvin cycle take place
Stroma
Calvin cycle has three steps what are they
Co2 fixation
Co2 reduction
Regeneration of RuBP
Co2 reduction does what
It reduces Co2
Co2 becomes CH20
Possible by energy and electrons supplied by ATP and NADPH from light reactions
Regeneration of RuBP does what
Reforms RuBP by
Going through the cycle 3 times allows G3P exit, which uses 5 of them for 3 cycle
Creating RuBP
Why is the Calvin cycle important
G3P ca be used to for many other molecules like
Fatty acid and glycerol for plant oil
Glucose
Fructose
Starch and cellulose
Photosynthesis vs cellular respiration
Both use cellular respiration
Mitochondria
Break down glucose
O2 to co2
Only plants do photosynthesis
Chloroplast
Build glucose
Co2 to o2
Both use electron transport chain and chemiosmosis to produce ATP
DNA and RNA are polymers of what
Nucleotides
They are polymers (nucleic acids) of monomers (nucleotides)
DNA nucleotides have 4 nitrogenous bases
Adenine
Thymine
Cytosine
Guanine
DNA vs RNA
DNA deoxyribonucleic acid
RNA Ribonucleic acid
Uses ribose instead of deoxyribose
Uracil instead of thymine
Single strand
Who discovered dna
James Watson and Francis crick worked out 3D structure of DNA based off of Rosalind Franklin
Double helix is dna structure
DNA structure
Double helix
Held by hydrogen bond
At
CG
DNA replication does what basically
DNA to DNA (identical to parent strand)
It is semi conservative
Old strand makes new strand
DNA helicase
Breaks unzip dna strand
DNA polymerase
Puts new strand of dna together
DNA ligase seal any breaks in sugar phosphate backbone
Gene expression is the process
Of using gene sequence to synthesize a protein
How many process is inside gene expression
Transcription
Translation
Transcription
Take place in nucleus
DNA to RNA
DNA serves as template for mRNA formation
Uses RNA Polymerase to create mRNA
starts transcribing when there is a certain nucleotide sequence called promoter
Terminator tells it to stop transcribing
Genetic code
Triplet code - each three nucleotide unit of mRNA molecule is a codon
mRNA codons
There are 64 different mRNA Codons
61 code for particular amino acids
Redundant code - codes of some amino acid have numerous code words
Protect against mutation
Co2 fixation has what happening
Co2 is attached to 5 carbon RuBP molecule
Happens 3 times
Results in 6Carbon molecule splits into two 3 carbon molecules
Done by RuBP carboxylase (stroma is full of this
Translation has 3 parts what are they
Initiation (needs energy)
Elongation (need energy)
Termination
Translation: initiation
Brings ribosome components together
Small ribosome sub unit
mRNA
Initiator tRNA (attach to start codon AUG)
Large ribosomal subunit
3 binding site for tRNA
P (peptide) site
A (amino acid) site
E (exit) site
Translation: elongation
Process by which polypeptide increases in length
Steps in elongation
tRNA approaches A site (A tRNA w attached peptide is at P site)
tRNA binds to A site
Peptide bond forms to attach the new amino acid to the peptide chain
Ribosome moves forward, empty tRNA exits the E site and tRNA carries new amino acid to the ribosome
Translation: termination
Termination occurs at the stop codon
Causes formation of 2 ribosomal subunits
What are the differences btw introns and Exons
Introns are removed
Exons are used bc they have DNA that can make protein
They are the expressed portion of genes
Gene mutations are what
Permanent change in the sequence of bases in dna
What causes gene mutations
Error in replication
Mutagens
Transposons
Viruses
Error in replication
DNA replication errors are rare for mutation source
Bc DNA polymerase proof reads to catch error
Mutagens are what
Environmental influences that can cause mutation
DNA repairs enzyme constantly to monitor and repair
Transposons are what
Specific DNA sequences that move within or between chromosomes
(Weird occurrence were they get cut and spliced and out at diff spots)
Viruses can do what to cause mutagen
They insert their dna into host chromosome
Can cause HPV - leads to cervical cancer
3 type of point mutations are
Substitution
May change specific amino acid (change a nucleotide)
Insertion (frameshift)
Insert nucleotide
Deletion (frameshift)
Delete nucleotide