Plasticwares (CHAPTER 1)

Question 1

clear, rigid, not autoclavable, and for disposable wares

Answer 1

Polystyrene

Question 2

Not recommended for use with acids, aldehydes, ketones, ethers, hydrocarbons or essential oils.

Answer 2

Polystyrene

Question 3

Alcohols and bases can be used but not to be stored longer than 24 hours.

Answer 3

Polystyrene

Question 4

a unique group of resins with relatively inert chemical properties.

Answer 4

Polyolefins

Question 5

Two types of polyolefins

Answer 5

-polyethylene
-polypropylene

Question 6

A type of polyolefin that is chemically resistant to most substances except for aldehydes, amines, ethers, hydrocarbons and
essential oils

Answer 6

Polyethylene

Question 7

translucent and flexible; not autoclavable; more expensive

Answer 7

Conventional Polyethylene

Question 8

• Has the same chemical resistant as polyethylene that is Translucent and rigid; autoclavable but absorbs pigment and tends to become discolored.

Answer 8

polypropylene

Question 9

• Used for screw-cap closures

Answer 9

polypropylene

Question 10

✓ Resin that has excellent chemical resistance to almost all chemicals in the lab.
✓ Clear, translucent and flexible; autoclavable

Answer 10

Teflon Fluorocarbon Resins

Question 11

Used for stopcocks, wash bottles and tubings, for cryogenic experiments and work at high
temperatures over extended periods ( -270℃to 255℃)

Answer 11

Teflon Fluorocarbon resins

Question 12

✓ Translucent and flexible; nontoxic, clear plastic of modified PVC ( polyvinylchloride), autoclavable;
✓ used for tubings

Answer 12

Tygon

Question 13

• Very susceptible to damage by most chemicals. Resistant to water, aqueous salts and inorganic acids
  for a long period.

Answer 13

Polycarbonate

Question 14

Twice as strong as polypropylene ( from -100⁰C to 160⁰C)

Answer 14

Polycarbonate

Question 15

• Very clear and rigid; autoclavable
• Used for carboys , ideal for centrifuge tubes and graduated cylinders.

Answer 15

Polycarbonate

Question 16

Used for most bottles and tubings

Answer 16

Polyvinyl chloride

Question 17

what does cryogenic mean?

Answer 17

performed at a very low temp.

Question 18

other name of Teflon Fluorocarbon resins

Answer 18

Fluorinated ethylene propylene

Question 19

What is the standard measurement of Cuvets?

Answer 19

13mm x 100mm

Question 20

type of cuvet that is used to contain acidic solutions

Answer 20

Soft

Question 21

type of cuvet that is used for alkaline solutions

Answer 21

hard/borosilicate

Question 22

which type of cuvet is used for wide range settings (300-700 nm or 380-700 nm) as to wavelength settings

Answer 22

glass

Question 23

which type of cuvet is for short wavelength setting and has a UVR below 340nm

Answer 23

quartz

Question 24

Disadvantages of Plasticwares

Answer 24

1. Evaporation of solutions – increase in concentration
2. Absorb dyes/pigments – decreased reaction accuracy
3. Color is difficult to describe

Question 25

STERILIZATION OF HIGH QUALITY PLASTICWARE
1. Autoclaving:

Answer 25

121C at 15psi for 15-20 mins.

Question 26

STERILIZATION OF HIGH QUALITY PLASTICWARE
2. Chemical sterilization

Answer 26

Benzalkonium chloride

Question 27

STERILIZATION OF HIGH QUALITY PLASTICWARE
3. Gas sterilization

Answer 27

Ethylene oxide

Question 28

they are usually used to transfer volumes of 20 mL or less

Answer 28

pipets

Question 29

is designed to transfer a KNOWN volume of liquid

Answer 29

transfer pipet

Question 31

Routine washing

Answer 31

20% @HNO3 & acid dichromate solution

Question 32

Blood clots

Answer 32

10% NaOH

Question 33

New pipet

Answer 33

5% HCl & 5% HNO3

Question 34

Metal ion determination

Answer 34

20% HNO3

Question 35

Grease

Answer 35

50% KOH or any organic solvent

Question 36

Permanganate stains

Answer 36

50% HCl or 1%Fe2SO4 in 25%H2SO4

Question 37

Bacteriologic glassware

Answer 37

2-4% cresol

Question 38

Iron determination

Answer 38

1:2 - dilution of concd. HCl
1:3 - concd. HNO3