Plasticwares (CHAPTER 1) Flashcards
clear, rigid, not autoclavable, and for disposable wares
Polystyrene
Not recommended for use with acids, aldehydes, ketones, ethers, hydrocarbons or essential oils.
Polystyrene
Alcohols and bases can be used but not to be stored longer than 24 hours.
Polystyrene
a unique group of resins with relatively inert chemical properties.
Polyolefins
Two types of polyolefins
-polyethylene
-polypropylene
A type of polyolefin that is chemically resistant to most substances except for aldehydes, amines, ethers, hydrocarbons and
essential oils
Polyethylene
translucent and flexible; not autoclavable; more expensive
Conventional Polyethylene
- Has the same chemical resistant as polyethylene that is Translucent and rigid; autoclavable but absorbs pigment and tends to become discolored.
polypropylene
- Used for screw-cap closures
polypropylene
✓ Resin that has excellent chemical resistance to almost all chemicals in the lab.
✓ Clear, translucent and flexible; autoclavable
Teflon Fluorocarbon Resins
Used for stopcocks, wash bottles and tubings, for cryogenic experiments and work at high
temperatures over extended periods ( -270℃to 255℃)
Teflon Fluorocarbon resins
✓ Translucent and flexible; nontoxic, clear plastic of modified PVC ( polyvinylchloride), autoclavable;
✓ used for tubings
Tygon
- Very susceptible to damage by most chemicals. Resistant to water, aqueous salts and inorganic acids
for a long period.
Polycarbonate
Twice as strong as polypropylene ( from -100⁰C to 160⁰C)
Polycarbonate
- Very clear and rigid; autoclavable
- Used for carboys , ideal for centrifuge tubes and graduated cylinders.
Polycarbonate
Used for most bottles and tubings
Polyvinyl chloride
what does cryogenic mean?
performed at a very low temp.
other name of Teflon Fluorocarbon resins
Fluorinated ethylene propylene
What is the standard measurement of Cuvets?
13mm x 100mm
type of cuvet that is used to contain acidic solutions
Soft
type of cuvet that is used for alkaline solutions
hard/borosilicate
which type of cuvet is used for wide range settings (300-700 nm or 380-700 nm) as to wavelength settings
glass
which type of cuvet is for short wavelength setting and has a UVR below 340nm
quartz
Disadvantages of Plasticwares
- Evaporation of solutions – increase in concentration
- Absorb dyes/pigments – decreased reaction accuracy
- Color is difficult to describe
STERILIZATION OF HIGH QUALITY PLASTICWARE
1. Autoclaving:
121C at 15psi for 15-20 mins.
STERILIZATION OF HIGH QUALITY PLASTICWARE
2. Chemical sterilization
Benzalkonium chloride
STERILIZATION OF HIGH QUALITY PLASTICWARE
3. Gas sterilization
Ethylene oxide
they are usually used to transfer volumes of 20 mL or less
pipets
is designed to transfer a KNOWN volume of liquid
transfer pipet
Routine washing
20% @HNO3 & acid dichromate solution
Blood clots
10% NaOH
New pipet
5% HCl & 5% HNO3
Metal ion determination
20% HNO3
Grease
50% KOH or any organic solvent
Permanganate stains
50% HCl or 1%Fe2SO4 in 25%H2SO4
Bacteriologic glassware
2-4% cresol
Iron determination
1:2 - dilution of concd. HCl
1:3 - concd. HNO3
