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CLINICAL CHEM > PHLEBOTOMY > Flashcards

PHLEBOTOMY Flashcards

AGREVIEW KA PANGAASIM

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1
Q

collection of minute sample of blood through capillary puncture

A

microsampling

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2
Q

depth of puncture for infants up to 1 year of age

A

2.4 mm

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3
Q

puncture site for infants up to 1 year of age

A
  • plantar surface of the big toe
  • median or lateral side of the heel
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4
Q

depth of puncture for adults

A

3.1 mm

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5
Q

puncture site for adults

A
  • Middle or ring finger
  • Puncture must be slightly off-center, perpendicular to the fingerprint
  • Margin of the earlobe
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6
Q

collection of a greater volume of blood from the veins or arteries

A

macrosampling

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7
Q

Collection of arterial blood for blood gas analysis or blood pH determination

A

arterial puncture

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8
Q

common sites for arterial puncture

A
  • radial artery
  • brachial artery
  • femoral artery
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9
Q
  • Collection of venous blood
  • Most frequent site: Antecubital vein of the forearm
A

venipuncture

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10
Q

venipuncture site for infants up to 18 months

A
  • external jugular vein
  • superior longitudinal sinus
  • temporal vein
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11
Q

venipuncture sites for 18 months to 3 years

A
  • popliteal vein
  • femoral vein
  • long saphenous vein
  • ankle vein
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12
Q

venipuncture sites for 3 years to adulthood

A
  • veins on the antecubital fossa
  • wrist vein
  • veins on the dorsal of hands and feet
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13
Q

methods of performing venipuncture

A
  • open system
  • closed system
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14
Q

– use of syringes
- The hub of the needle is color-coded, corresponding to its gauge

A

open system

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15
Q

The lower the gauge, the bigger the bore of the needle

A

open system

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16
Q

use of an evacuated system (evacuated tube, two-way needle and adapter)
- Evacuated tubes are equipped with a hemogard (color-coded in accordance to the additive
present)
- Multiple sampling can be carried out

A

closed system

17
Q

liquid portion of clotted blood

A

serum

18
Q

liquid portion of anti-coagulated blood

A

plasma

19
Q

: preparation involves the removal of proteins from any biological
specimen to prevent direct colorimetric interference by the formation of zwitterions at isoelectric pH where
proteins exhibit maximum precipitation and minimum solubility

A

protein - free filtrate

20
Q

: preparation involves the removal of proteins from any biological
specimen to prevent direct colorimetric interference by the formation of ____________ at isoelectric pH where
proteins exhibit maximum precipitation and minimum solubility

A

zwitterions

21
Q

: preparation involves the removal of proteins from any biological
specimen to prevent direct colorimetric interference by the formation of zwitterions at ____________ where
proteins exhibit maximum precipitation and minimum solubility

A

isoelectric point

22
Q

methods of PFF preparation

A

a. physical method
b. chemical method

23
Q

what are the physical methods of PFF preparation?

A
  1. heat
  2. ultracentrifuge
24
Q

acid method in the ppf preparation

A
  1. Folin - Wu
  2. Hayden’s method
  3. Van Slyke
  4. TCA (Tricarboxylic acid cycle)
25
Q

base method in the PFF method

A
  • Nelson-Somogyi
26
Q

contamination of specimen

A

❖ residual detergent in tubes
❖ Plasticizers in IV tubing and tube stoppers
❖ Cork stoppers & glass tubes
❖ Lead analysis: use lead-free, acid-washed containers

27
Q

rate of glycolysis at ref temp

A

2mg/dL

28
Q

rate of glycolysis at room temp

A

7mg/dL

29
Q

what specimens can be centrifuged?

A

lipemic

30
Q

NPO meaning

A

nothing by mouth

31
Q

how many hours of fasting is needed for glucose determination

A

6-8 hours

32
Q

how many hours of fasting is needed for

A

10 - 12 hours

33
Q

what test should be done to determine wether the ulnar artery can provide collateral circulation to the hand after the radial artery puncture

A

modified Allen test

34
Q
A

CLINICAL CHEM (7 decks)

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