Plasmids

Question 1

Conjugation

Answer 1

The process of transferring plasmids between bacteria to allow the sharing of genetic material

Question 2

Transfection

Answer 2

Introducing plasmids into cells

Question 3

Constructs / vectors

Answer 3

Plasmids created in a lab

Question 4

Transgenic bacteria

Answer 4

Bacteria that have recombinant plasmids

Question 5

Plasmids

Answer 5

Extrachromosomal, circular, stable and easy to manipulate dsDNA

Question 6

Plasmid diagram

Answer 6

The arrows indicates the genes that code for proteins and their direction indicates the direction of transcription

Question 7

Origin of replication

Answer 7

Makes more copies of plasmids as bacteria grow and divide

Question 8

Antibiotic resistance gene

Answer 8

Enables growth in the presence of a specific antibiotic

Question 9

Inserted gene

Answer 9

Encodes a particular protein, it is found between restriction sites

Question 10

Promoter

Answer 10

Promotes gene transcription

Question 11

Restriction sites

Answer 11

Cut by restriction enzymes to paste another gene

Question 12

Multiple cloning sites

Answer 12

DNA segment that contains several restriction sites

Question 13

Electroporation

Answer 13

Electric pulses to open the membranes of bacterial cells

Question 14

Restriction enzymes

Answer 14

Cut DNA and plasmid to help form a recombinant molecule and used to destroy foreign DNA

Question 15

DNA ligase

Answer 15

Forms the recombinant molecule by connecting the sticky ends

Question 16

Green fluorescent protein

Answer 16

Used to tag a gene to make it visible