Pics or didn't happen Flashcards

Micribiology pictuers

1
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S. mansoni egg

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2
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Plasmodium falciparum

Rings may possess one or two chromatin dots. They may be found on the periphery of the RBC (accolé, appliqué) and multiply-infected RBCs are not uncommon. Ring forms may become compact or pleomorphic depending on the quality of the blood or if there is a delay in making smears. There is usually no enlargement of infected RBCs.

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3
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Plasmodium falciparum

Rings may possess one or two chromatin dots. They may be found on the periphery of the RBC (accolé, appliqué) and multiply-infected RBCs are not uncommon. Ring forms may become compact or pleomorphic depending on the quality of the blood or if there is a delay in making smears. There is usually no enlargement of infected RBCs.

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4
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Gametocyte of plasmodium falciparum

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5
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Schizont p.falciparum

(rare to see in blood except in severe cases)

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6
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P. ovale

Developing trophozoites of P. ovale are compact with little vacuolation. Infected RBCs are often slightly enlarged and may exhibit fimbriation and Schüffner’s dots. Pigment is less-coarse and diffuse

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7
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P. vivax

Figure B: Trophozoite of P. vivax in a thin blood smear. Note the amoeboid appearance, Schüffner’s dots and enlarged infected RBCs.

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7
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P. ovale

Figure C: Trophozoite of P. ovale in a thin blood smear. Note the fimbriation and Schüffner’s dots.

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8
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Figure A: Trophozoite of P. vivax in a thick blood smear.

Developing trophozoites of P. vivax become increasingly amoeboid, with tenuous pseudopodial processes and large vacuoles. Schüffner’s dots are visible with proper staining. Pigment tends to be fine and brown. Infected RBCs are usually noticeably larger than uninfected RBCs.

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9
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Amastagotes of leishmania

Amastigotes of Leishmania are spherical to ovoid and measure 1-5 µm long by 1-2 µm wide. They possess a large nucleus, a prominent kinetoplast, and a short axoneme, the last of which is rarely visible by light microscopy. The organisms reside in macrophages of the host and can be found throughout the body.

Amastigotes of Leishmania spp. are morphologically indistinguishable from those of Trypanosoma cruzi

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10
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Just some normal macrophages for reference

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11
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Normal blood smear for reference

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12
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Figure A: Toxoplasma gondii tachyzoites, stained with Giemsa, from a smear of peritoneal fluid obtained from a laboratory-inoculated mouse.

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13
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Figure A: Toxoplasma gondii cyst in brain tissue stained with hematoxylin and eosin

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14
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Entamoeba Histolytica

Figure A: Cyst of E. histolytica/E. dispar in an unstained concentrated wet mount of stool. Notice the chromatoid bodies with blunt, rounded ends (arrow)

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15
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Entamoeba Histolytica

Figure A: Cyst of E. histolytica/E. dispar in an unstained concentrated wet mount of stool. Notice the chromatoid bodies with blunt, rounded ends (arrow)

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16
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Promastigotes Leish

Infective stage

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17
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Ascaris egg

unfertilised

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18
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Ascaris lumbricoides egg

Fertilised

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19
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Taenia Egg

See a tyre think Taenia

(cannot differentiate between solium and saginata)

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20
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Hookworm Egg

Thin shell - hatches early, does not need to be durable. Hatches to rhabiditiform –> filariform larvae (Infectious stage)

Segementum ovum

Eg
Necator Americanus
Ancylostoma Duodenialis

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21
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Trichuris Trhicura

tea tray
big

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22
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Fasciola Hepatica

Treatment of triclabendazole 7/7 BD

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23
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Diphyllobothrium Latium

Fish tape worm

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24
Which diseases is this a vector for?
Aedes Dengue Yellow fever Zika Chickungunya
25
Which diseases is this a vector for?
Culex JEV WNV
26
Which diseases is this a vector for?
Anopheles Malaria
27
Schistosoma Japonicum
28
Schistosoma Haematobium
29
Metacyclic trypamastigote (infectious stage) T.Cruzi T cruzi and brucei - pretty similar looking. Larger kinetoplast in cruzi
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Cryptococcus neoformans India ink stain
31
P. malariae trophozoite band form
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P. malariae schizont Rosette
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Wuchereria bancfroti microfilaria Sheath pink Empty tail Nuceli relatively distinct
34
Gabon/Cameroon
Loa Loa MF Ghost sheath Nuclei towards tail flattened
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Indonesia
Brugia Timori Empty head (Tim has an empty head) More nuclei in tail
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Onchocerciasis Slab head (swollen empty head) Empty tail Onchocerciasis skin snip
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Name each MF
38
Name the vector
Sandfly Lutzomyia Leishmaniasis
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Name the vector
Black fly Simulium spp Onchocerciasis
40
Name the vector
Red fly Chrysops sp loa loa
41
Name the vector
Scabies mite Sarcoptes scabei
42
Name the vector
Triatome bug (kissing bug)
43
Name the vector
The humble rat flea Xenopsylla cheopis
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Genus Disease caused
Ambylomma AFTS Rickettsia Africa
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Genus Disease caused
Hyalomma hard tic CCHF
46
Name Disease caused
Schistosome Japnonicum Schistosomiasis Animal reservoir - cats/dogs/pigs in philipines
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Taenia Solium
48
Taenia Saginta
49
Diphyloobothrium latum
50
Hymenolopis Nana
51
Hymeolopis diminuta
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Diphylidium cani
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Trichuris Trichura
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Hymenolepis nana egg
55
Onchocerca Volvulus
56
Paragonimus spp
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4 complications
Entermoeba histolytica 1. Amoebic abcess - most commonly in the liver 2. Ameoboma - chronic inflammatory mass, single or multiple, most commonly developing in the ileocaecal region, presenting as and acute/subactue obstruction or causing an intussusception. 3. Haemorrhage - resulting in anaemia or shock 4. Post dysenteric ulcerative colitis - mimicking classical ulcerative colitis. usually resolves slowly without specific treatment
58
Risk in pregnancy
Schizont of p.falciparum maternal anaemia, increased risk of severe malaria, retardation of foetal growth, stillbirth
59
Hookworm rhabiditiform larvae(L1) Looks very similar to strongyloides
60
Tsetse fly (glossina species) Vector for African trypanosomiasis
61
Toxocaris canii egg
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Clonorchis Siniensis The eggs are oval shaped with a convex operculum that rests on visible “shoulders” at the smaller end of the egg Praziquantel
63
Pullex irritans
64
Xenopsylla Cheops
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Ctenocephalides canis or C. felis (this one is felis)
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Giardia duodenalis trophozoites
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Giardia duodenalis cyst
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Proctoscolex Echinoccous Granulosus Hydatid disease PAIR procedure
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Rhabiditiform Larvae Strongyloides Stercoralis No long buccal tube like Hookworm
70
Hookworm L1 larvae (rhabiditiform larvae) 250-300um long eg Ancylostoma duodenalis or Nectar Americanus Long buccal tube parallel lines - different to strongy
71
Hookworm L3 Larvae (filariform larvae) 500-700um long eg Ancylostoma duodenalis or Nectar Americanus Strongy has notched tail
72
Strongyloides Stercoralis 500-700um long L3 larvae Notched tail
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Thick film Plasmodium falciparum trophozoites
74
Plasmodium Falciparum Schizont RBC is normal size Multiple merozoites 2/3 full RBC
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Plasmodium ovale schizont RBC is enlarged Merozoites 4-16, average is 8 Cell is 2/3 full
76
Plasmodium vivax schizont RBC is enlarged Lots of merozoites - 12-24 Cell is full
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P.Ovale gamete - macrogamete Generally less enlargement than P.Vivax
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P.ovale gamete - microgamete
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P.vivax gamete - macrogamete
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P.vivax gametocyte
81
P.malariae gametocyte Gametocytes of P. malariae are compact and tend to fill the host RBC. There is no enlargement of the infected RBC and sometimes there is a reduction in size. The cytoplasm stains blue and the chromatin pink to red. Abundant dark pigment may be scattered throughout the cytoplasm.
82
Trichenella Spiralis Encysted larva Nurse cell
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Paragonimus westermani egg
84
Clonorchis Siuensis
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Trypomastigote - Trypanosome Brucei (Gambiense or rhodiense) Small kinetoplast = brucei Big/massive/mega = South America
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Cryptosporidium cyst
87
Sequestration of RBC infected with plasmodium falciparum Pathophysiology for cerebral malaria
88
Cyclops - freshwater Dracularis Mediensis (guinea worm)
89
Infective stage of - Fasciola, Clonorchis, Fasciolopsis buski, paragonimus
metacercariae Essentially in all other trematodes which are not schistososma