Physiology 10 Flashcards
What is the alveolar gas equation?
PAO2 = PIO2 - PICO2 / RQ
RQ = respiratory quotient
RQ depends on mix of dietary intake but is commonly quoted as 0.8
What is the A-a gradient?
What is its normal value?
A-a gradient = PAO2 - PaO2
Under normal conditions this is <2 kPa
What factors affect O2 carrying capacity in the blood?
Hb
SaO2
PaO2
How is HbO2 carrying capacity calculated?
HbO2 carrying capacity = [Hb] x 1.39 x SaO2/100
How is dissolved oxygen in the blood calculated?
Dissolved O2 = 0.023 x PaO2 ml/100ml
What is the equation for calculating total blood O2 content?
O2 content = [Hb] x 1.39 x SaO2/100 + (0.023 x PaO2)
Regarding the HbO2 dissociation curve what is the P50 at pH 7.4?
3.5kPa
How is oxygen delivery to tissues (DO2) defined?
DO2 = blood O2 content x CO
Regarding the HbO2 dissociation curve what is the P75 at pH 7.4?
Why is this number important?
5.3 kPa
This is a typical value for mixed venous blood
How is VO2 derived?
VO2 = CO x (Ca02 - CvO2)
For a ‘normal’ person, what is the O2 content of 100% saturated blood?
Approx 20ml/100ml
Using normal values to calculate, what is a normal VO2?
VO2 = 5000 x (20 - 15)/100
= 250 ml/min
Outline the concept of tissue hypoxia
When intracellular PO2 is insufficient to sustain normal aerobic metabolism for cellular functions
How is tissue hypoxia classified?
- Hypoxic hypoxia (low PO2)
- Anaemic hypoxia (reduced or dysfunctional Hb)
- Ischaemic hypoxia (low CO or vascular abnormality)
- Histotoxic hypoxia (inability of cells to utilise available oxygen)
What are the phases of cellular metabolism?
Phase 1: Production of 2-carbon compounds
Phase 2: Citric acid cycle
Phase 3: Electron transport chain
Outline processes relevant to phase 1 metabolism
Glycolysis (Glucose 6c -> 2x Pyruvate 3c) - Produces 2 NADH2+ and [net] 2 ATP (4 -2)
Glycolysis occurs in the cytoplasm
Oxidative decarboxylation (Pyruvate 3c + CoA -> Acetyl CoA 2c + CO2) - Produces 2 NADH2+ Oxidative decarboxylation occurs in the mitochondria
Beta-oxidation of free fatty acids in mitochondria produces Acetyl CoA
Oxidation of amino acids produces Pyruvate, Acetyl CoA and other intermediates
Outline the key points of Phase 2 metabolism
Citric Acid Cycle
- Acetyl CoA combines with Oxaloacetate to form citrate
- Citrate goes through a series of reactions producing intermediary compounds, energy-containing compounds and CO2
- The cycle ends with oxaloacetate, allowing the cycle to start again.
- Per glucose molecule, 2 cycles will produce 2 ATP, 6 NADH2+, 2 FADH2 and 4 CO2
Outline the key points of phase 3 metabolism
Electron Transport Chain
- Reduced energy-containing compounds are re-oxidised producing electrons and energy, used to phosphorylate ADP -> ATP
- Each NADH2+ produces 3 ATP in the ETC
- Each FADH2+ produces 2 ATP in the ETC
- Oxygen is the final electron acceptor in the chain
What is the breakdown of net ATP production from one glucose molecule during aerobic respiration?
Glycolysis: 8 ATP (2 ATP + 2 NAHD2+)
Oxidative decarboxylation: 6 ATP (2 NADH2+)
Citric acid cycle: 24 ATP (2 ATP + 6 NADH2+ + 2 FADH2)
What is the breakdown of net ATP production from one glucose molecule during anaerobic respiration?
Glycolysis: 2 ATP
The NADH2+ is used in the metabolism of pyruvate to lactate.
NAD+ and FAD are not re-formed so the citric acid cycle cannot continue
Lack of oxygen means the ETC cannot operate
How long can the body’s supply of ATP last?
Approx 90 seconds
What is the lowest possible mitochondrial PO2 compatible with oxidative phosphorylation?
0.4 kPa
What are the main mechanisms by which cellular hypoxia causes loss of function?
- Fall in ATP levels
- Fall in pH
How does the body compensate for hypoxia?
- Early / Late
- Local / Ventilatory / Cardiovascular
Early local: Changes to HbO2 affinity, vasodilatation
Early ventilatory: Hypoxic (<7 kPa) / hypercarbic response
Early CV: Vasoconstriction, tachycardia (to ^CO/MAP)
Late: Polycythaemia (detectable in 3-5 days)
How does the cerebral circulation respond to hypoxia?
PO2 <7 kPa leads to exponential increases in cerebral blood flow due to vasodilatation