phylloplane Flashcards
What is the rhizosphere ?
below ground habitat colonised by microbes
What is the phyllosphere ?
Aerial plant habitat (plant structures above ground) colonised by microbes
What are epiphytes?
Microbes
What is the on line in situ monitoring mechanism ?
Robbins- type devices
What is the off line pilot scale monitoring mechanism?
several km rigs
e.g Nancy, kuopio, kempton
what are the off line lab mechanisms ?
flow cell, chemostats, annular reactors, propella
What genes are responsible for transcription of core structures of nitrogen fixers?
flavonoids/ nod genes
What are the features of argobacterium?
ca2+ dependent adhesin, EPS, cellulose fibres
What plant disease give evidence of subsurface invasion via stoma
By studying citrus cranker.
What is GFP bacteria ?
strain of bacteria engineered to produce green fluorescent protein
What does EDIC/EF combined with EF live tracking GFP salmonella on leaves show?
- start off with bacteria in cell margins all over leaf
- within 2 hours bacteria in cell margins and stomatal apertures
How is the mutant csgB salmonella different?
mutant strain partly lacking curly fimbriae
but does not show difference in attachment
How does salmonella bacteria move across the leaf surface ?
any free water on surface allows them to seek refuge in cell margins and somata ( less hostile enviro)
biofilm forms
How are salad leaves adapted?
- bind very tightly to resist removal by washing
- form biofilms to adhere better and shield from chemical sanitisers e.g chlorine
What are the temperatures of the two sites biofilms are studied: mains disruption supplies and building plumbing systems
Mains 4-20 C
Building plumbing 20-60C
What are MBEC high-throughput assays used for?
determine efficiency of antimicrobial agents on polymicorbial biofilms
Biofilms established on pegs under batch conditions
what is the difference between lab flow cell and removable flow cell?
lab flow cell = not robust, low shear, slow flow
Removable = robust, higher shear
What does ATR-FTIR Ge crystal flow cell measure?
vibrations
which Robbins device has higher pressure. in line or lab?
in line
What are the possible disadvantages of annular reactor models?
swirling current effect (eddy)
x and y gradients
What are the possible disadvantages for chemostat models?
shear forces not completely controlled
but well mixed (more than annular reactors)
What are the conditions for chemostat and annular models?
pH, nutrients, open flow system, no leaks
control hydraulic retention through flow rate
what is the name of the model which combines the best of the chemostat and annular reactors?
Propella
