FISH

Question 1

What is LNA-FISH?

Answer 1

locked nucleic acid FISH

Question 2

What is PNA- FISH?

Answer 2

peptide nucleic acid

Question 3

What is CARD-FISH?

Answer 3

catalysed reporter deposition

Question 4

What is CLASI- FISH?

Answer 4

combinational labelling and spectral imaging

Question 5

What is MAR-FISH?

Answer 5

micro auto radiography

Question 6

What is ISRT-FISH?

Answer 6

in situ reverse transcription FISH

Question 7

Describe LNA-FISH

Answer 7

mixed with DNA or RNA
increase sensitivity and specificity

Question 8

Describe PNA-FISH

Answer 8

-ve sugar phosphate backbone is replaced by non-charged peptide backbone
shorter probe length
don’t have to remove biofilm from enviro

Question 9

Describe CARD-FISH

Answer 9

• signal increased by horseradish-peroxidase labelled nucleotides
• 20x brighter signal
• more cell permeabilisation steps for larger enzyme-labelled oligonucleotides
• large stain = accessibility decreased

Question 10

Describe CLASI-FISH

Answer 10

uses combinational probes to label bacterial in mixed populations
spectral imaging so don’t need to determine colours by eye

Question 11

Describe MAR-FISH

Answer 11

uptake radioactively labelled substrates into cell
cells can’t be permeabilised

Question 12

Describe ISRT-FISH

Answer 12

studies metabolically active groups and control gene expression