CRISPR Flashcards
What does class 1 type 1 do?
- unwinds comp dsDNA
- cas3
- cas form around CRISPR RNA
What does class 1 type 3 do?
- cleaves comp RNA
- cas 10
- target associated ssDNA cleavage
- cylcic oligodenylate synthesis
What does class 1 type 4 do?
- cascade not yet understood
What does class 2 type 2 do?
cas 9
cleaves comp dsDNA
Results in a guide RNA with loops
What are the domains involved in class 2 type 2 and type 5?
RuuC for cleaving
PI for PAM recognition
What does class 2 type 5 do?
cas12
cleaves comp dsDNA
What does class 2 type 6 do?
cas 13
non specific cleavage of comp ssRNA
Causes suicide of the cell
What domain is activated by a conformational change in class 2 type 6?
2 HEPN
What does csm3 cause?
inactivation of HD and Palm domains of cas10 (specific cleavage of phage)
What does csm3 cause?
inactivation of HD and Palm domains of cas10 (specific cleavage of phage)
What break type does cas9 cause when used for gene editing ?
blunt double stranded break
What break type does cas12 cause when used for gene editing ?
staggered DNA double stranded break
precise
What is homology-directed repair (HDR) ?
Gene editing by double or single stranded oligodeoxynucleotide donor templates which have homology arms to cut at target site
What is used to repair double stranded breaks?
non-homologous end joining (NHEJ) mediated repair
What carried out single base editing ?
cas9
