Phlebotomy Highlights <3 Flashcards
phlebotomy
vein cutting
phlebotomist
blood collector
Laboratory analyses of a variety of specimens are used for three important clinical purposes:
- diagnostic testing
- therapeutic assessments
- monitoring
Define the purposes of each test
- diagnostic testing: to figure out what is wrong with the patient
- therapeutic assessments: to develop the appropriate therapy or treatment of the medical condition
- monitoring: to make sure the therapy or treatment is working to alleviate the disease or illness
Technology has enabled laboratory testing to be performed closer to the point of care POC. Define POC
point-of-care (POC) testing: refers to tests and procedures that are performed at patient’s bedside or at the “point of care”. these tests are not sent to a lab in a remote location, rather they are rapid methods designed to produce quick results
FDA stands for?
Food and Drug Administration
an agency that regulates and oversees clinical laboratories; which itself is regulated by the federal government
CMS stands for?
Centers for Medicare & Medicaid Services
an agency that regulates and oversees clinical laboratories; which itself is regulated by the federal government
FDA regulates all clinical laboratories through what?
Through CLIA 1988: Clinical Laboratory Improvement Amendments of 1988
CBC stands for?
complete blood count
CLIA categorizes lab tests according to level of complexity of the procedure and risk involved for the patient if errors are made in performing or interpreting the test. These three include:
- waived tests
- tests of moderate complexity
- tests of high complexity
Elaborate on each test.
- waived tests: easiest to perform and least susceptible to error and least risky to patients. Examples include urinalysis, urine pregnancy tests, blood glucose screening tests, blood detection from stool sample, etc. These tests are commonly done in ambulatory settings, on hospitals near the beside and in other remote locations.
- tests of moderate complexity: simple to perform but involve more risk to patient if results are inaccurate. Such include white and red blood cell counts, hemoglobin, hematocrit, blood chemistries and urine cultures.
- tests of high complexity: tests that are complex to perform and may allow reasonable risk of harm to patient if results are inaccurate. These may require sophisticated instrumentation and oversight by a pathologist or PhD level scientist. Such include molecular analyses, bone marrow evaluations, immunoassays, flow cytometry, cytogenetics analysis and electrophoresis.
When are blood specimen tubes labeled? What information does it include?
At the bedside next to the patient. Information needed include: name, dob, date/time (usually in military time)
Six Sigma
Six Sigma is an example of quality framework; method designed to improve process performance by reducing variation, improving quality and enhancing financial performance while improving customer satisfaction.
List examples of inability to collect a blood specimen
Examples of when inability to collect a blood specimen would occur include when:
- collection attempts are unsuccessful (no more than 2 sticks)
- the patient is unavailable
- the patient refuses to have blood drawn
Quality control
quality control measures are functions of everyday practice in clinical laboratories that make the testing process more accurate and precise
What should you do before leaving a patient?
After the procedure but before leaving the room, always THANK the patient for their cooperation.
STAT verbal request
STAT: immediate
What is the difference between ethics and bioethics?
Ethics: moral standards of behavior or conduct that govern an individual’s actions
Bioethics: refers to the moral issues or problems that resulted because of modern medicine, clinical research and or technology; refers to life and death issues such as abortion, when a patient should be allowed to die and who receives organ donations
Malpractice
defined as professional negligence. Improper or unskillful care of a patient by a member of the healthcare team, or any professional misconduct or unreasonable lack of skill
Negligence
Failure to act or to perform duties according to the standards of the profession; violation of a duty to exercise reasonable skill and care in performing a task
Standard of care
Specific standard of care to protect patients; it is a measuring stick representing the conduct of the average health care worker in the community; the community has become a national community as a result of national laboratory standards and requirements
Informed consent
voluntary permission given by a patient to allow touching, examination and or treatment by health care providers
Implied consent
occurs when the patient’s nonverbal behavior indicates agreement; exists when immediate action is required to save a patient’s life or to prevent permanent impairment of the patient’s health
What does CLIA stand for and what is its purpose?
Clinical Laboratory Improvement Amendments ensure the quality and accuracy of laboratory testing
Define infection
the condition in which the body is invaded with pathogenic (disease causing) microorganisms such as bacteria, fungi, viruses and parasites
What does BBPs stand for and what are they? What are examples of them?
BBPS: blood-borne pathogens describes any infectious microorganism present in blood and other body fluids and tissues that can cause infectious diseases.
Such include: hepatitis A, B, C, D and E; HIV (AIDS), syphilis, malaria, human T-cell lymphotrotrophic virus (HTLV) types I and II
What is CDC the acronym for?
Centers for Disease Control and Prevention - oversees the investigation and control of various diseases, especially those that are communicable and threaten the US population
What are universal precautions and who were they established by?
Universal precautions established through OSHA (Occupational Safety and Health Administration), an agency of the US Department of Labor, OSHA requires employers to provide measures that will protect workers exposed to biological hazards including training to avoid BBP exposures.
Designed to prevent transmission of all infectious agents in the health care setting
What are the 3 components that make up the “chain of infection”?
- source
- mode of transmission
- susceptible host
What ratio do you need to clean phlebotomy trays or specimen collection areas with?
Clean phlebotomy trays at least once a week with a 1:10 bleach solution
Clean specimen collection area with a decontaminating 1:10 bleach solution
What does PASS stand for?
Proper Use of Fire Extinguishers
Pull pin
Aim nozzle
Squeeze handle
Sweep side to side
What does RACE stand for?
Health care workers in response to possible fire emergencies should RACE: Rescue Alert Confine Extinguish
MSDS
Material safety data sheets
Because various chemicals are needed in healthcare facilities especially in the clinical lab department and because chemicals may pose health or physical hazards, OSHA amended WHAT to include health care facilities?
HCS: Hazard Communication Standard AKA HazCom
HazCom standard requires chemical manufacturers to supply MSDS for their chemicals
NFPA Rating System p146
Understand the blue.red.yellow and white boxes in the diamond indicate; what type of hazard? What do their numbers indicate?
Blue: Health Hazard 4 Deadly 3 Extreme Danger 2 Hazardous 1 Slightly hazardous 0 Normal material
Red: Fire hazard 4 Below 73F 3 Below 100F 2 Above 100F 1 Not exceeding 200F 0 Will not burn
Yellow: Reactivity/Instability 4 May detonate 3 Shock & heat 2 Violent chemical change 1 Unstable if heated 0 Stable
White: Specific hazard ACID- acid ALK- alkali COR- corrosive OXY- oxidizer P- polymerization Hazard sign- radioactive W- use no water
What is the periosteum?
In general, bones consist of several layers covered by a membrane, the periosteum. The periosteum contains blood vessels that bring blood from inside the bone to the outer layer.
When do vessels get smaller and larger? When they head towards…?
Vessels get smaller and smaller as they move away from the heart (arteries) and then get larger again as they return toward the heart (veins)
What is the largest artery and veins called?
Aorta: largest artery
Veins: venae cavae
What are highly oxygenated vessels that carry blood away from the heart?
Arteries
Arteries appear brighter red in color because of the oxygen
What is blood carried toward the heart by?
Veins
All veins except the pulmonary veins contain deoxygenated blood and under the skin appear bluish in color and have thinner walls than arteries
What are the three types of veins used for venipuncture and what differentiates them?
The antecubital area of the forearm is most commonly used for venipuncture.
The median cubital vein (middle) is best for venipuncture because it is generally the largest and best anchored vein.
Other acceptable ones are the basilic vein (left bottom) and cephalic vein (upper outer right) p219.
Differentiate the 2 types of blood and how they differentiate in terms of bleeding?
Arterial blood is bright red in color (due to high O2 content) and since pressure is higher in arteries, bleeding is usually quicker.
Venous blood is dark red in color (because it lacks O2) and bleeding occurs in a steady flow.
What are circulating blood cells classified as?
Red blood cells/RBCs/erythrocytes as well as white blood cells/WBCs/leukocytes
What color tubes do RBCs go into?
purple/lavender
What does SST stand for?
Serum separator
Erythropoiesis
Production of red blood cells, millions of which normally are formed and destroyed daily
Erythropoietin
Hormone produced in the kidney that triggers erythropoiesis
What does a pink tube top test for?
Blood typing
What is the most common blood type?
Type O
What are white blood cells also known as?
WBCs or leukocytes
What does CBC stand for?
complete blood count
go into lavender tube tops
What is the liquid portion of blood and lymph called?
Plasma
If a blood specimen is allowed to clot, the resulting liquid portion is called what?
What kind of clot are the blood cells meshed in?
Resulting liquid portion is serum while the blood cells are meshed into a fibrin clot.
Hemostasis
Complex series of processes in which platelets, plasma, and coagulation factors interact to control bleeding while at the same time maintaining circulating blood in the liquid state
Injury to a vessel wall begins the basic steps in hemostasis
What types of medication can increase bleeding due to one of its properties being a blood thinner?
Aspirin & Coumadin (warfarin)
Anticoagulants
Used for patients to inhibit blood clot formation
What system has a close and interrelated connection with the cardiovascular system? What does this system consist of? What are the 3 main functions of the system?
The lymphatic system, which consists of lymph, lymphocytes, lymph vessels, lymph nodes, tonsils, the spleen, bone marrow and the thymus gland.
The three main functions are:
- maintain fluid balance in the tissues by filtering blood and lymph fluid
- provide a defense and immunity against disease through the lymphocytes
- distribute nutrients and hormones into the bloodstream, remove waste, an absorb fats and other substances from the digestive tract
Edema
swellings
Venipuncture
blood collection from a vein
Skin puncture
blood collection from a finger and/or an infant’s heel
What on tubes should be monitored continuously?
expiration dates
STAT means?
immediate
Collection Tubes: what additive is added to clotted blood/serum gray/red or clear
no additive (discard tube)
Collection Tubes: what additive is added to clotted blood/serum red
None or clot activator in plastic tube
Collection Tubes: what additive is added to clotted blood/serum red/black
Clot activator and polymer barrier
Collection Tubes: what additive is added to whole blood/plasma light blue
sodium citrate (3.2% or 3.8%)
Collection Tubes: what additive is added to whole blood/plasma lavender (purple)
EDTA
Collection Tubes: what additive is added to whole blood/plasma gray
sodium flouride and potassium oxalate or sodium flouride and Na2EDTA
Collection Tubes: what additive is added to whole blood/plasma green
lithium heparin, sodium heparin or ammonium heparin
Collection Tubes: what additive is added to whole blood/plasma royal blue
K2EDTA-sterile tube for toxicology and nutritional studies
Collection Tubes: what additive is added to whole blood yellow
Sodium polyanetholsulfonate (SPS) or acid citrate dextrose (ACD)
What additive is in the yellow topped tubes for blood culture specimen collections in microbiology?
Sodium polyanethol sulfonate (SPS)
What needle should you use to collect blood cultures with?
Butterfly needle
Coagulation procedures such as PT and APTT are done in what colored topped vacuum tubes?
Light blue topped vacuum tubes that contain sodium citrate
If light blue tubed top is underfilled, coagulation results will be erroneously prolonged