Pharmacometabolomics (Anas Lecture)

Question 1

Define “metabolomics”.

Answer 1

Comprehensive ID & quantification of all metabolites within a biological system

Question 2

Define “pharmacometabolomics”.

Answer 2

Comparisons of a patient’s metabolic signature before and after drug exposure

Question 3

What can the field of Metabolomics be applied to?

Answer 3

-Biomarker Discovery

-Drug Discovery & Development

-Environmental / Genetic Variation

Question 4

Describe the “Biomarker Discovery Workflow”.

Answer 4

-Start by conducting Global / Fingerprinting analysis of a patient group to determine candidate biomarkers.

-Propose question & quantify various candidate biomarkers.

-Develop an understand of the role of said biomarker & validate it.

Question 5

Number one reason why Metabolomics is favored (on occasion) over Genomics / Transcriptomics / Proteomics?

Answer 5

Much more dynamic & rapid responding to stimulus than others!

Question 6

Most prominent tech used in Metabolomic studies?

Answer 6

LC-MS

Question 7

T or F: Metabolites have close biological proximity to phenotype.

Answer 7

True!

Question 8

Challenges in Metabolomics?

Answer 8

-Diverse LMW compounds

-Wide Concentration Range

-Not specific to singular pathways

-Heterogenous PC properties

-Genetic / Env. stimuli