Peritonitis and Intra-Abdominal Infection Flashcards

1
Q

What is Peritonitis and how would it present?

A

Inflammation of tye peritoneum /serosal lining of the abdominal cavity

  • can be generalised/diffuse
  • may be localised/abscess infection
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2
Q

What’s the difference between primary, secondary and tertiary peritonitis?

A

Primary: (spontaneous): diffuse bacterial infection w/o loss of GI tract integrity. Rare, ass. with patients with liver disease

Secondary: acute infection resulting from loss of GI tract integrity or from infected viscera. Most common. Related to visceral pathology and post-surgical infection

Tertiary: recurrent oinfection of peritoneal cavity following inadequate initial therapy. Often fromdefective immunity

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3
Q

What causes peritonitis?

A

Bacteria! From GI tract to peritoneum!

Poly microbial infection

  • >1 species involved
  • synergistic infection
  • bact. are reflective of where they came from
  • Hospital acquired infections the only way to have one species!!

Bacterial:

  • Enterobacteriaceae
    • ​E.coli, klebsiella, enterobacter
  • Anaerobes
    • GNB: B. fragilis
  • Enterococci
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4
Q

Where are the sources of microbial pathogens that cause Peritonitis?

A

Mainly all the GI tract!!

  • Stomach/duodenum
    • 103-106 bacteria ml-1
    • aerobes and facultative anaerobes
  • Jejunum/ileum
    • 104-107 bacteria ml-1
    • Transition from aerobes and facultative anaerobes to more anaerobes
  • Colon
    • 1011-1013 bacteria ml-1
    • anaerobes and facultative anaerobes
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5
Q

WHats the Route of Transmission?

A

Secondary: From GI tract → peritoneum via a perforation

  • Appendicitis (ruptured appendix) Why surgery is so dangerous
  • Diverticulitis (rupture of inflammed diverticulum)
  • stomach/duodenal ulcer
  • infection/abscess of other visceral organs
  • Pelvic inflammatory disease
  • surgery/trauma
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6
Q

Risk Factors of Peritonitis?

A

Primary: Liver disease, portal vein hypertension and ascites

Secondary: Appendicitis, diverticulitis and ulcers

Tertiary: immune deficiencies, previous primary or secondary peritonitis.

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7
Q

What happens in the peritoneum when the bacteria get to the peritoneum?

A
  1. Gain entry
  2. Bacteria not cleared
    • Not all phagocytosed by macrophage
    • not all able to be contained in a fibrin clot
    • Clearance not effective in the presence of nutrients (eg; Hb) and necrotic tissue
  3. Bacterial Proliferation
  4. Inflammation
    • fluid exudate in peritoneal cavity
    • dillution of antibacterial factors
    • may lead to hypovolemia
  5. Abscess formation
    • fibrin deposit traps bacteria
    • may prevent phagocytosis and other antimicrobial access
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8
Q

A CT/US will show?

A

Fluid accumulation and inflammation

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9
Q

How would you use diagnostic microbiology?

A
  1. Aspirate pus
    • foul smelling
  2. Gram’s stain of the pus from the abscess
    • gram-negative rods
    • possibly gram positive cocci
    • probably over one type

For Anaerobic and Aerobic cultures

  • Culture from pus
  • anaerobic transport swabs: vital to not kill the bacteria before we can test it!
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10
Q

Whats the issue with bacteria testing?

Why does isolation require

A

THey are fastidious anddie easily, are difficult to islate and are over looked.

These are often present iin mixed infection

Isolation requires appropriate methods of collection: aseptic aspiration, transportation and cultivation of specimens.

Use selective agar, bile aesculin agae, anaerobic

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11
Q

If it’s gram Negative?

A

GLC used to be used, now mass spectrometry and PCR are more often used?

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12
Q

What is this?

A
  • Half blood agar, half bile esculin

Blood Agar: stuff growing, not haeolytic and no halos present that you may see with other bacterias (eg; strep)

Bile-Esculin agar: Bile inhibits growth in anything not from GI. Esculin is a carb cleaved by enzymes that bacteroides, that changes colour to black when cleaved

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13
Q

Polymicrobial infections are usually from….

A

Synergy between usually B. fragilis and E. coli

_B. Fragilis: p_olysaccharide casule Bacteroides fragilis is an obligately anaerobic, Gram-negative, rod-shaped bacterium

  • antiphagocytic capsule and LPS
  • Capsule elicits deposition of fibrin
    • abscess formation
  • Can degrade complement by proteases
  • Obligate anaerobes, reduced O2 toxicity; SOD, catalase (both iron containing proteins)

E. coli: good at getting iron, in mixed infections; E. coli is a haem binding protein, so that HBP can be intercepted by B. fragilis

Therefore when dealing with the infection you need to kill BOTH

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14
Q

Why do you treat with broader spectrum antibiotics?

A

As this is usually always a polymicrobial infection, therapy needs to target all possible organisms.

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15
Q

Treatment of peritonitis?

A
  1. Treat symptoms
    • fluids, pain relief
    • removal/drainage of pus guided by US/CT
  2. Treat source
    • find cause and control sepsis origin
    • removal/drainage of pus guided by CT/US
    • removal of Dead tissue
    • corrective surgery to repair leak
  3. Treat the microbial cause
    • empiric antimicrobial therapy
    • broad spectrum AB
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16
Q

What is the broad spectrum triple-therapy used to treat peritonitis?

A

E. coli Treated by: aminoglycosides, 4th gen cephalosporin

Anaerobes (eg; B. fragilis): metronidazole

Enterococcus: ampicillin

this targets the three main groups of pathogens!!

But you don’t want to kill all the bacteria in the gut bc this can be dangerous long-term!!!

Single therapy is less toxic for patients with liver/kidney disease

17
Q

How long should we be treating the microbial causes?

A
  • ~1 week (sometimes 4-6) until resolved, not to long to avoid damage to gut flora
    • danger of C. difficile infection with broad spectrum is a worry!
      *
18
Q

Metronidazole

A

Good antibiotic against bacteria

  • Exact mode of action is UNCLEAR
  • Inhibits DNA synthesis
  • Only works on anaerobes and some proteazoa
  • INEFFECTIVE against aerobic and facultatively anaerobic bacteria
19
Q

what’s going on?

A
  • Appendicits: surgical emergency. Inflamed appendix has ‘leaked’ some pus and ruptures during removal
  • Aspiration of pus: sent to lab for culturing, gram-staining
  • GI damage repaired/stable
  • monitor patient
  • lots of broad scheme ABs
  • FLuids and pain relief
  • Monitor bowel
20
Q

What to ask?

A
  • History: past history of diverticulitis
  • RLQ pain, no bowel sounds, fever
  • DIverticulum burst and leaked → peritonitis , as weel as ass. blockage of GI tract (either contributary or the primary cause of the burst).
  • Do everything as before (treat immediate symptoms, remove bad tissue, then antibiotics to completlely clear)