Peripheral blood cell culture and harvest

Question 1

An atmosphere containing __ CO2 is necessary to maintain the proper pH for cell growth

Answer 1

5%

Question 2

PHA stimulates ___ to divide

Answer 2

T-cells

Question 3

After the addition of PHA, transformation occurs within ____ hours and peak mitotic activity occurs between ___ hours of culture

Answer 3

12-24 hours, 60-70 hours

Question 4

Pokeweed mitogen stimulates ___ to divide

Answer 4

both T and B-cells

Question 5

2 main methods to obtain elongated chromosomes

Answer 5

Cell synchronization and restriction of chromosome condensation

Question 6

In cell synchronization the block is often applied at ____

Answer 6

S-phase

Question 7

In cell synchronization the block is usually released after ___

Answer 7

5-5.5 hours

Question 8

Treatment for mitotic arrest

Answer 8

Colcemid or colchicine

Question 9

Methotrexate blocks cell division at S phase by…

Answer 9

Disrupting synthesis of thymine

Question 10

MTX or FUdR block can be bypassed by …

Answer 10

addition of thymidine

Question 11

Most commonly used hypotonic solution :

Answer 11

0.075M KCl