Long term cell culture and harvest Flashcards
1
Q
Growth media is complete media plus…
A
10-20% serum
2
Q
Freezing media contains:
A
5-10% DMSO or glycerol
3
Q
Difference between vertical and horizontal laminar flow hoods?
A
Horizontal laminar flow hoods blow air over sample and onto the worker (no worker protection, should not be used)`
4
Q
How long can you store Complete Media at 4C?
A
2-3 weeks
5
Q
Phenol red media: magenta
A
too basic
6
Q
Phenol red media: yellow
A
too acidic
7
Q
Mycoplasma
A
submicroscopic, not affected by most antibiotics, can cause deletions/rearrangements/cell death.
Detect by staining with Hoechst 33258
8
Q
Maintenance media…
A
higher pH and 1-3% serum
