Perio Microbiology Flashcards
Are perio pathogens communicable?
Yes! - Lee et al. 2006 - BANA positive caregiver is strongest predictor of BANA positive plaque in child.
35x more likely than a BANA neg caregiver/child
Do you need to brush the gums?
No - Desquamation (epithelial shedding) Occurs twice/day - BUT, not on tongue or under RPD - brush tongue/under RPD
What are the different types of deposits that accumulate on teeth?
Materia Alba
Dental Plaque
Calculus
What is Materia Alba
White - cheese like substance Accumulation of Bacteria Salivary glycoproteins Desquamated epithelial cells Food debris Not as organized as Dental Plaque Easily removed with water-spray
What is Dental Plaque
Yellowish-gray resilient substance
Composed of bacteria in a matrix of salivary glycoproteins and extracellular polysaccharides
Considered a Biofilm
Cant be removed with spray
What is Calculus
Mineralized Dental Plaque
covered by a layer of unmineralized
How does plaque progress from nothing to a mature plaque?
Acquired Pellicle Transport of microbes to pellicle Adhesion (reversible) Attachment (strong) Co-Adhesion (micro-colonies) Colonization (mature plaque)
What is the acquired pellicle composed of?
Peptides Proteins Glycoproteins Phosphoproteins Mucins Keratins Statherins
How does adhesion develop?
Acquired pellicle develops immidiately
After 2hrs - equilibrium of deposition/shedding bacteria
1) Transport - Brownian motion, sedimentation of bacteria on surface
2) Initial adhesion - Van der Waals forces, Hydrophobicity
3) Strong Attachment - Microbe surface Adhesins bind Glycoproteins/proteins/polysaccharides on pellicle
4) Co-adhesion and micro-colony formation
What is the most dominant bridge bacteria? what complex is it a part of?
Fusobaterium Nucleatum
Orange
What complexes are primary colonizers?
Secondary?
Yellow, Blue, Purple, No complex (Actinomyces Naeslundi, A. oris)
Green, Orange, Red (and Aa(b))
What bacteria are a part of the Yellow complex?
Streptococcus sanguis S. intermedius S. mitis S. gordonii S. oralis
What bacteria are a part of the Blue complex (not a defined complex in socransky)
Actinomyces species (naeslundii)
What bacteria are a part of the Purple complex?
Villinella Parvula
Actinomyces Odontiliticus
What bacteria are a part of the Green complex?
Capnocytophaga concisus C. ochracea C. gingivalis C. sputigena Eikenella corodens A. a. (a)
What bac. are a part of the Orange complex
Prevetella intermedia Prevetella nigresens Petpostreptococcus micros Campylobacter rectus C. gracilis C. showae Fusobacterium nucleatum F. periodonticum Eubacterium nodatum Streptococcus constellatus
What is the Red complex
P gingivalis
T forsythia
T. denticola
what is the No complex?
A.a. (b)
S. noxia
What bacteria is most indicated in effecting pregnancy outcomes and colorectal cancer?
F. nucleatum
What things affect disease activity?
not just the bacteria, but individual host susceptibility and the presence of interacting bacterial species (fusobacterium)
What is Socransky’s modified Koch’s postulates?
The bacteria should cause disease in animal models
Should have a virulence factor that is identifiable and found to cause periodontal destruction
Should illicit a cellular or humoral immune response
Should be found in increased quantity in diseased sites
Should be found in lower/no quantity in resolved/treated sites
What is the Orange complex associated with clinically?
increasing PD
What is the Red complex associated with clinically?
Deepest PD when all 3 are present
Shallowest when none are present
BOP
Ximénez-Fyvie et al. 2000
With Socransky
Took supra and sub G samples
Most common in both supra and sub is actinomyces
Only difference between health and disease is the proportion of actinomyces to orange and red complex
SupraG plaque and be a reservoir to spread and re-infect subG sites
What study described the necrotizing periodontal lesion? What are they?
Listgarten 1965
The Bacterial zone
The Neutrophil Rich Zone
The Necrotic Zone
The Zone of Spirochetal infiltration
