PEANUT 1 Flashcards
All of the following inhibit growth hormone secretion, except:
A. Glucose loading
B. Insulin, nutritional deficiencies
C. Thyroxine deficiency
D. Amino acids, sleep, exercise
D. Amino acids, sleep, exercise
Mega:
A. 10^3
B. 10^-3
C. 10^6
D. 10^-6
C. 10^6
Micro:
A. 10^3
B. 10^-3
C. 10^6
D. 10^-6
D. 10^-6
1:3S
- One observation exceeds 3 SD from the target value
- Three observations exceed 1 SD from the target value
- Imprecision or systematic bias
- Not recommended
A. 1 and 3
B. 2 and 4
C. 1 and 4
D. 1, 3 and 4
A. 1 and 3
8:1S
- Range between two observations exceeds 4 SD
- Eight sequential observations for the same QC sample exceed 1 SD
- Imprecision
- Bias trend
A. 1 and 3
B. 2 and 4
C. 1, 3 and 4
D. 2, 3 and 4
B. 2 and 4
Basic unit for mass:
A. Mole
B. Milligram
C. Gram
D. Kilogram
D. Kilogram
Basic unit for length:
A. Second
B. Millimeter
C. Meter
D. Kilometer
C. Meter
Overall capacity to transport bile:
A. Serum bilirubin level
B. Ratio of direct and total bilirubin
C. Serum ALP
D. Serum bile acids
A. Serum bilirubin level
Overall patency of the biliary ducts:
A. Serum bilirubin level
B. Ratio of direct and total bilirubin
C. Serum ALP
D. Serum bile acids
D. Serum bile acids
Capacity to conjugate bilirubin and secrete bile:
A. Serum bilirubin level
B. Ratio of direct and total bilirubin
C. Serum ALP
D. Serum bile acids
A. Serum bilirubin level
Capacity to conjugate bilirubin; quantity of hemoglobin turnover:
A. Serum bilirubin level
B. Ratio of direct and total bilirubin
C. Serum ALP
D. Serum bile acids
B. Ratio of direct and total bilirubin
Patency of the biliary ducts, hepatocellular metabolism of bilirubin:
A. Serum bilirubin level
B. Ratio of direct and total bilirubin
C. Serum ALP
D. Serum bile acids
B. Ratio of direct and total bilirubin
Abnormality of bile duct epithelium:
A. Serum bilirubin level
B. Ratio of direct and total bilirubin
C. Serum ALP
D. Serum bile acids
C. Serum ALP
Hepatocellular damage and necrosis:
A. Serum albumin
B. Serum ALP
C. Serum aminotransferase levels
D. Blood ammonia
C. Serum aminotransferase levels
Capacity to synthesize protein:
A. Serum albumin
B. Blood ammonia
C. Blood urea
D. Serum bile acids
A. Serum albumin
Lipemic or icteric specimen for coagulation studies:
A. Use an optical instrument
B. Use a mechanical instrument
C. Both of these
D. None of these
B. Use a mechanical instrument
Effect of prolonged tourniquet application to concentration of vWF and Factor VIII:
A. Increased
B. Decreased
C. Variable
D. Undetermined
A. Increased
Hemoglobinopathies due to ABNORMAL MOLECULAR STRUCTURE:
- Sickle cell anemia
- Sickle cell trait
- HbC disease or trait
- Alpha thalassemia
A. 1 and 3
B. 2 and 4
C. 1, 2 and 3
D. 1, 2, 3 and 4
C. 1, 2 and 3
Hemoglobinopathies due to ABNORMAL RATE OF SYNTHESIS:
- Alpha thalassemia
- Sickle cell anemia
- Beta thalassemia
- Sickle cell trait
A. 1 and 3
B. 2 and 4
C. 1, 2 and 3
D. 1, 2, 3 and 4
A. 1 and 3
Which of the following statistical terms reflects the best index of precision when comparing two CBC parameters?
A. Mean
B. Median
C. Coefficient of variation
D. Standard deviation
C. Coefficient of variation
Most commonly encountered autoimmune hemolytic anemia:
A. Drug-induced AIHA
B. Cold AIHA
C. Warm AIHA
D. Mixed-type AIHA
C. Warm AIHA
OSMOTIC FRAGILITY TEST: SANFORD METHOD
Normal RBCs, INITIAL hemolysis occurs in which tube:
A. Tube 1
B. Tube 17
C. Tube 22
D. Tube 25
C. Tube 22
OSMOTIC FRAGILITY TEST: SANFORD METHOD
Normal RBCs, COMPLETE hemolysis occurs in which tube:
A. Tube 1
B. Tube 17
C. Tube 22
D. Tube 25
B. Tube 17
Which of the following is contained in the primary granules of the neutrophil?
A. Lactoferrin
B. Myeloperoxidase
C. Histamine
D. Alkaline phosphatase
B. Myeloperoxidase
What reagents are used in the PT test?
A. Thromboplastin and sodium chloride
B. Thromboplastin and potassium chloride
C. Thromboplastin and calcium
D. Actin and calcium chloride
C. Thromboplastin and calcium
A modification of which procedure can be used to measure fibrinogen?
A. PT
B. APTT
C. Thrombin time
D. Fibrin degradation products
C. Thrombin time
Which test result would be normal in a patient with dysfibrinogenemia?
A. Thrombin time
B. APTT
C. PT
D. Immunologic fibrinogen level
D. Immunologic fibrinogen level
Factor XII deficiency is associated with:
A. Bleeding episodes
B. Epistaxis
C. Decreased risk of thrombosis
D. Increased risk of thrombosis
D. Increased risk of thrombosis
The most common subtype of classic von Willebrand’s disease is:
A. Type 1
B. Type 2A
C. Type 2B
D. Type 3
A. Type 1
The clot-based method of Clauss, a modification of the TCT, is the recommended procedure for estimating the functional fibrinogen level.
The operator adds reagent bovine THROMBIN to dilute PPP, catalyzing the conversion of fibrinogen to fibrin polymer.
In the fibrinogen assay, the thrombin reagent concentration is 50 NIH units/mL.
The PPP to be tested is diluted _____ with Owren buffer.
A. 1:2
B. 1:5
C. 1:10
D. 1:100
C. 1:10
What clotting factors (cofactors) are inhibited by protein S?
A. V and X
B. Va and VIIIa
C. VIII and IX
D. VIII and X
B. Va and VIIIa
Which of the following tests is most likely to be abnormal in patients taking aspirin?
A. Platelet morphology
B. Platelet count
C. Bleeding time
D. Prothrombin time
C. Bleeding time
The Bethesda assay is used for which determination?
A. Lupus anticoagulant titer
B. Factor VIII inhibitor titer
C. Factor V Leiden titer
D. Protein S deficiency
B. Factor VIII inhibitor titer
Heparin-induced thrombocytopenia (HIT) results from:
A. Antibodies to heparin
B. Antibodies to platelets
C. Antibodies to PF4
D. Antibodies to heparin-PF4 complex
D. Antibodies to heparin-PF4 complex
A plasma sample submitted to the lab for PT testing has been stored for 25 hours at 4°C. The PT result is shortened. What is the most probable cause?
A. Factor VII deficiency
B. Activation of factor VII due to exposure to cold temperature
C. Lupus inhibitor
D. Factor X inhibitor
B. Activation of factor VII due to exposure to cold temperature
The four essential functions of a manager are:
A. Staffing, decision making, cost analysis, evaluating
B. Directing, leading, forecasting, implementing
C. Planning, organizing, directing, controlling
D. Innovating, designing, coordinating, problemsolving
C. Planning, organizing, directing, controlling
Which order of events should be followed at the conclusion of a laboratory worker’s shift in order to prevent the spread of bloodborne pathogens?
A. Remove gloves, disinfect area, wash hands, remove lab coat
B. Disinfect area, remove gloves, remove lab coat, wash hands
C. Disinfect area, remove gloves, wash hands, remove lab coat
D. Remove gloves, wash hands, remove lab coat, disinfect area
B. Disinfect area, remove gloves, remove lab coat, wash hands
The material safety data sheets (MSDSs) for hazardous chemicals address which of the following conditions?
A. Physical characteristics of the chemical
B. Safe handling and storage of the chemical
C. Specific health hazards associated with the chemical
D. All of these options
D. All of these options
Which monochromator specification is required in order to measure the true absorbance of a compound having a natural absorption bandwidth of 30 nm?
A. 50-nm bandpass
B. 25-nm bandpass
C. 15-nm bandpass
D. 5-nm bandpass
D. 5-nm bandpass
A dialysis patient is positive for both hepatitis B surface antigen and hepatitis B surface antibody. The physician suspects a laboratory error. Do you agree?
A. Yes; the patient should not test positive for both HBsAg and HBsAb
B. No; incomplete dialysis of a patient in the core window phase of hepatitis B infection will yield this result
C. No; it is likely the patient has recently received a hepatitis B booster vaccination and could have these results
D. Perhaps; a new specimen should be submitted to clear up the confusion
C. No; it is likely the patient has recently received a hepatitis B booster vaccination and could have these results
An initial and repeat ELISA test for antibodies to HIV-1 are both positive. A Western blot shows a single band at gp160. The patient shows no clinical signs of HIV infection, and the patient’s CD4 T-cell count is normal. Based upon these results, which conclusion is correct?
A. Patient is diagnosed as HIV-1-positive
B. Patient is diagnosed as HIV-2-positive
C. Results are inconclusive
D. Patient is diagnosed as HIV-1-negative
C. Results are inconclusive
A transplant patient began to show signs of rejection 8 days after receipt of the transplanted organ, and the organ was removed. What immune elements might be found in the rejected organ?
A. Antibody and complement
B. Primarily antibody
C. Macrophages
D. T cells
D. T cells
From the following, identify a specific component of the adaptive immune system that is formed in response to antigenic stimulation:
A. Lysozyme
B. Complement
C. Commensal organisms
D. Immunoglobulin
D. Immunoglobulin
A wound (skin lesion) specimen obtained from a newborn grew predominantly β-hemolytic colonies of gram-positive cocci on 5% sheep blood agar. The newborn infant was covered with small skin eruptions that gave the appearance of a “scalding of the skin.” The gram-positive cocci proved to be catalase positive. Which tests should follow for the appropriate identification?
A. Optochin, bile solubility, PYR
B. Coagulase, glucose fermentation, DNase
C. Bacitracin, PYR, 6.5% salt broth
D. CAMP, bile-esculin, 6.5% salt broth
B. Coagulase, glucose fermentation, DNase
An isolate recovered from a vaginal culture obtained from a 25-year-old female patient who is 8 months pregnant is shown to be a gram-positive cocci, catalase negative, and β-hemolytic on blood agar. Which tests are needed for further identification?
A. Optochin, bile solubility, PYR
B. Bacitracin, CAMP, PYR
C. Methicillin, PYR, trehalose
D. Coagulase, glucose, PYR
B. Bacitracin, CAMP, PYR
A gram-positive bacillus was isolated from a wound specimen and had the following characteristics: double zone of β hemolysis, lecithinase positive, lipase negative, spot indole negative. What is the most likely identification of this organism?
A. Clostridium perfringens
B. Clostridium ramosum
C. Clostridium septicum
D. Clostridium tetani
A. Clostridium perfringens
Microscopically, numerous smooth, thin-walled, club-shaped, multiseptate (2 to 4 μm) macroconidia are seen. They are rounded at the tip and are borne singly on a conidiophore or in groups of two or three. MICROCONIDIA ARE ABSENT, spiral hyphae are rare, and chlamydoconidia are usually numerous.
A. Epidermophyton floccosum
B. Microsporum canis
C. Trichophyton mentagrophytes
D. Trichophyton rubrum
A. Epidermophyton floccosum
Which is the most appropriate nonselective medium for recovery of mycobacteria from a heavily contaminated specimen?
A. Löwenstein–Jensen agar
B. Middlebrook 7H10 agar
C. Petragnani’s agar
D. American Thoracic Society medium
C. Petragnani’s agar
Acid-fast positive bacilli were recovered from the sputum of a 79-year-old man who had been treated for pneumonia. Which of the following test reactions after 3 weeks of incubation on Löwenstein–Jensen agar are consistent with Mycobacterium tuberculosis?
A. Niacin +, Nitrate reduction +, Photochromogen negative
B. Niacin negative, Optochin +, Catalase +
C. PYR +, Urease +, Bacitracin +
D. Ampicillin resistant, Penicillin resistant
A. Niacin +, Nitrate reduction +, Photochromogen negative
Which of the following has been recognized in postinfectious complications of a Campylobacter jejuni infection?
A. Guillain-Barré syndrome
B. Chronic pulmonary disease
C. Encephalitis
D. Endocarditis
A. Guillain-Barré syndrome
Valuable combination in therapy and follow-up in patients with germ cell tumors of the testes:
A. CEA and PSA
B. CEA and AFP
C. AFP and β-HCG
D. CEA and CA-125
C. AFP and β-HCG
Small protozoa that have been missed on wet mounts or concentration methods are often detected with:
A. India ink
B. Iodine
C. Giemsa stain
D. Trichrome stain
D. Trichrome stain
Demonstrates the largest platelets seen and is also referred to as giant platelet syndrome:
A. Alport syndrome
B. Bernard-Soulier syndrome
C. May-Hegglin anomaly
D. Wiskott-Aldrich syndrome
B. Bernard-Soulier syndrome
Characterized by the presence of large platelets and the presence of Döhle-like bodies in the granulocytic leukocytes:
A. Alport syndrome
B. Bernard-Soulier syndrome
C. May-Hegglin anomaly
D. Wiskott-Aldrich syndrome
C. May-Hegglin anomaly
Disorder that exhibits giant platelets and thrombocytopenia: TURGEON
A. Alport syndrome
B. Bernard-Soulier syndrome
C. May-Hegglin anomaly
D. Wiskott-Aldrich syndrome
A. Alport syndrome
Demonstrates the smallest platelets seen:
A. Alport syndrome
B. Bernard-Soulier syndrome
C. May-Hegglin anomaly
D. Wiskott-Aldrich syndrome
D. Wiskott-Aldrich syndrome
The morphological characteristic(s) associated with the Chédiak–Higashi syndrome is (are):
A. Pale blue cytoplasmic inclusions
B. Giant lysosomal granules
C. Small, dark-staining granules and condensed nuclei
D. Nuclear hyposegmentation
B. Giant lysosomal granules
In flow cytometry, the term “gating” refers to:
A. Selection of a subpopulation of cells to count
B. Determining the fluorescent emission spectrumof cells of interest
C. Interference caused by binding of more than asingle antibody
D. Selecting the appropriate counting aperture
A. Selection of a subpopulation of cells to count
The two species of flies responsible for the transmission are Glossina palpalis and Glossina tachinoides:
A. Leishmania donovani
B. Leishmania tropica
C. Trypanosoma brucei gambiense
D. Trypanosoma brucei rhodesiense
C. Trypanosoma brucei gambiense
The two primary species of fly vectors responsible for transmitting are Glossina morsitans and Glossina pallidipes:
A. Leishmania donovani
B. Leishmania tropica
C. Trypanosoma brucei gambiense
D. Trypanosoma brucei rhodesiense
D. Trypanosoma brucei rhodesiense
Noting the appearance of plasma or serum can give important preliminary findings about lipid levels in the blood when it is collected from a fasting patient. When the specimen appears opaque and milky (lipemic), what is the approximate expected level of triglycerides in the sample?
A. Within the normal range; test is unaffected by meals.
B. From 200 to 300 mg/dL.
C. Greater than 600 mg/dL.
D. No preliminary findings can be made from observation of the serum.
C. Greater than 600 mg/dL.
Which of the following best describes the chemical principle of the protein reagent strip?
A. Protein reacts with an immunocomplex on the pad
B. Protein causes a pH change on the reagent strip pad
C. Protein accepts hydrogen ions from an indicator dye
D. Protein causes protons to be released from apolyelectrolyte
C. Protein accepts hydrogen ions from an indicator dye
Glucosuria not accompanied by hyperglycemia can be seen with which of the following?
A. Hormonal disorders
B. Gestational diabetes
C. Diabetes mellitus
D. Renal disease
D. Renal disease
The presence of dysmorphic red blood cells in the urine sediment is indicative of which of the following?
A. A coagulation disorder
B. Menstrual contamination
C. Urinary tract infection
D. Glomerular bleeding
D. Glomerular bleeding
Urinary casts are formed in which of the following?
A. Distal tubules and collecting ducts
B. Distal tubules and loops of Henle
C. Proximal and distal tubules
D. Proximal tubules and loops of Henle
A. Distal tubules and collecting ducts
A pale, frothy stool is indicative of which of the following?
A. Barium testing
B. Osmotic diarrhea
C. Steatorrhea
D. Excess carbohydrates
C. Steatorrhea
Stool specimens that appear ribbon-like are indicative of which condition?
A. Bile-duct obstruction
B. Colitis
C. Intestinal constriction
D. Malignancy
C. Intestinal constriction
In severe infections, yeasts may appear as:
A. Cocci-shaped
B. Small, refractile oval structures that may or may not contain a bud
C. Branched, mycelial forms
D. Pear-shaped flagellate with an undulating membrane
C. Branched, mycelial forms
Highly refractile spheres, usually with a dimpled center:
A. Hair fibers
B. Oil droplets
C. Pollen grains
D. Starch granules
D. Starch granules
GRANULAR, DIRTY, BROWN CASTS representing hemoglobin degradation products are associated with:
A. Cystitis
B. Acute pyelonephritis
C. Acute interstitial nephritis
D. Acute tubular necrosis
D. Acute tubular necrosis
Differentiation of WBC cast and epithelial cast:
A. Solubility in dilute HCl
B. Solubility at 60C
C. Supravital staining
D. Staining with Prussian blue
C. Supravital staining
IQ 200 system uses Auto Particle Recognition (APR) software that preclassifies urine particles in the photographs based on size, shape, texture and contrast into ____ categories.
A. 8
B. 10
C. 12
D. 15
C. 12
Bonding of antigen to antibody exists exclusively as:
A. Hydrogen bonding
B. Van der Waals forces
C. Electrostatic forces
D. Noncovalent bonding
D. Noncovalent bonding
Which of the following is a characteristic of fluorescent in situ hybridization (FISH)?
A. Semiconductor nanocrystals
B. Method of tagging antibodies with super paramagnetic particles
C. Technology based on two different 200-nm latex particles
D. Molecular cytogenetic technique
D. Molecular cytogenetic technique
Patient’s with Waldenstrom’s macroglobulinemia exhibit abnormally large amounts of:
A. IgM
B. IgG
C. IgE
D. IgA
A. IgM
Ten percent (10%) formalin is equivalent to what % paraformaldehyde?
A. 37%-40%
B. 10%
C. 4%
D. 1%
C. 4%
For good fixation it is recommended that the tissue be no larger than:
A. 2 cm square and 1-2 mm thick
B. 2 cm square and 3-4 mm thick
C. 3 cm square and 1-2 mm thick
D. 3 cm square and 3-4 mm thick
B. 2 cm square and 3-4 mm thick
10% formalin contains approximately what percentage of formaldehyde?
A. 4
B. 10
C. 37
D. 100
A. 4
A universal fixative used for routine purposes and which allows a broad spectrum of staining procedures is:
A. Zenker fluid
B. Zamboni PAF
C. 10% neutral buffered formalin
D. Carnoy solution
C. 10% neutral buffered formalin
A microwave oven can be used for fixation because it:
A. Causes cross-linking of proteins
B. Induces physical fixation
C. Increases tissue basophilia
D. Inactivates enzymes with beta-radiation
B. Induces physical fixation
Tissue sections were stained for the recommended time with an H & E procedure using Harris hematoxylin. A quality control check shows pale nuclear staining. A likely cause of this could be:
A. Too much alum mordant in the stock hematoxylin solution
B. Insufficient time in the acid differentiator
C. Too much time in the acid differentiator
D. Prolonged dehydration and clearing
C. Too much time in the acid differentiator
The connective tissue cells actively involved in wound healing are:
A. Plasma cells
B. Mast cells
C. Macrophages
D. Fibroblasts
D. Fibroblasts
The pigment commonly known as “wear and tear pigment” or “brown atrophy” is:
A. Hemofuscin
B. Ceroid
C. Lipofuscin
D. Hemosiderin
C. Lipofuscin
The staining method considered to be most sensitive and specific for copper is the:
A. Chloranilic acid
B. Rhodanine
C. Orcein
D. Aldehyde fuchsin
B. Rhodanine
Antibody molecules can belong to one of five immunoglobulin classes. The antibody class most frequently used in immunofluorescent and immunoenzyme staining is:
A. IgM
B. IgE
C. IgG
D. IgA
C. IgG
In immunoperoxidase staining of formalin-fixed tissue, enzyme pretreatment of antigens:
A. Enhances background staining
B. Reactivates antigens and enhances primary staining
C. Is needed to demonstrate all antigens
D. Has precise end-points
B. Reactivates antigens and enhances primary staining
A pathologic condition characterized by abnormal deposits of iron in the liver is called:
A. Hemachrosis
B. Hemochromatosis
C. Hemadostenosis
D. Hematotoxicosis
B. Hemochromatosis
A “Maltese cross” configuration is produced in tissue by polarization of:
A. Calcium oxalate
B. Uric acid
C. Talcum powder
D. Lipofuscin
C. Talcum powder
To prepare a 10% solution of formalin, which of the following amounts of water should be added to 100 mL of stock formaldehyde:
A. 1,000 mL
B. 900 mL
C. 450 mL
D. 10 mL
B. 900 mL
When the magnification can be changed without the need to refocus, the microscope objectives are said to be:
A. Parfocal
B. Binocular
C. Achromatic
D. Apochromatic
A. Parfocal
When performing immunohistochemistry (IHC), it is important to use which reagent, so that you can see where the staining from the antibody is in relation to the cellular structures within the tissue.
A. Absolute alcohol
B. Methanol
C. Primary stain
D. Counterstain
D. Counterstain
All of the following are immunologic functions of complement except:
A. Induction of an antiviral state
B. Opsonization
C. Chemotaxis
D. Anaphylatoxin formation
A. Induction of an antiviral state
Which specimen is the sample of choice to evaluate latent or tertiary syphilis?
A. Serum sample
B. Chancre fluid
C. CSF
D. Joint fluid
C. CSF
Which tests are considered screening tests for HIV?
A. ELISA, 4th generation, and rapid antibody tests
B. Immunofluorescence, Western blot, radioimmuno-precipitation assay
C. Culture, antigen capture assay, DNA amplification
D. Reverse transcriptase and messenger RNA(mRNA)
A. ELISA, 4th generation, and rapid antibody tests