PCR

Question 1

What is the role of PCR ?

Answer 1

Used to make multiple copies of a specific DNA segment .Involves preparing sample DNA , master mix with prieters and followed by detecting reaction product

PCR came into light in the 1980s by Kary B Mulls

Question 2

Advantages of PCR

Answer 2

Rapid 
Inexpensive 
Less time consuming
Simplicity 
dNtps sequence of target area of DNA does not need to be known in order to amplify that region

Question 3

Disadvantages of PCR

Answer 3

Works only in certain conditions

Question 4

Comparison Techniqe

Answer 4

Third Generation sequencing known as longer reads is a class of DNA sequencing methods currently under active development .

Works by reading nucleotide sequence at a single molecular level in contrast to existing methods

Requires breaking longer strands of DNA into smaller segment

Has higher error rates due to being an developing advanced technology but has the capability in producing longer segments of DNA

Question 5

PCR Denaturation

Answer 5

94 Degrees/95 degrees
Causes hygron bonds double strands to be separated into single strands.
15-30 seconds

Question 6

Annealing

Answer 6

60 *

Primers to binds to complementary sequences in DNA.
Run in opposite direction
Run for about 10 - 30 seconds

Question 7

Extension

Answer 7

72 *
Enables new DNA to made by a special enzyme known as TAQ polymerase adds DNA bases

Results in a new DNA stranded

DNA polymerise to attach to primers and copy DNA strands

Question 8

Forensic Medicine

Answer 8

• Where traces amount of DNA , in fluids such as blood or semen or in tissue such as hair.

Amplified by PCR

Analysed to see wheather DNA is identical to person suspected to commiting the crime

Question 9

Clinical Diagnoisis

Answer 9

Clinicians can detect infections by the aid viruses

HIV can be detected by PCR , causes destruction of patients .

Primers are made that are complementary of a particular gene on viral .

A sample taken from patient and track the progress of patient .