PCR Flashcards
1
Q
Denature __ Celsius
A
94, DNA unwinds into single strands
2
Q
Anneal __ Celsius
A
55, Anneal primers to DNA strands for new strand synthesis
3
Q
Extend __ Celsius
A
72, Extend new DNA from primers w/ DNTPS
4
Q
Components
A
DNA template
Primers
dNTPs (nucleotides)
Heat stable polymerase – Taq
Buffer sloth
