PCR

Question 1

Describe Polymerase Chain Reaction (PCR) and how actually it works, under different condition and procedures

Answer 1

1. The polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies (complete or partial) of a specific DNA sample.
2. This process occurs in three main stages:
   - Denaturation. Denaturation occurs when the reaction mixture is heated to 94℃ for about 0.5 to 2 minutes. …
   - Annealing. The reaction temperature is lowered to 54-60℃ for around 20-40 seconds. Here, the primers bind to their complementary sequences on the template DNA.
   - Elongation. At this step, the temperature is raised to 72-80℃

Question 2

. List about three requirement used in cell culture?

Answer 2

• culture media
• Inoculating loop
• Biosafety cabinate
• Vacuum waste flask
• Petri dishes or culturing flask
• Incubator
• Trypsin
• Tryptan blue
• ETHANOL 70%
• Conical tube
• hemocytometer